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Inhibiting staphylococcus aureus antibiotic resistance via photo-disassembly of membrane microdomains

  • Jie Hui
  • , Pu Ting Dong
  • , Lijia Liang
  • , Taraknath Mandal
  • , Junjie Li
  • , Erlinda R. Ulloa
  • , Yuewei Zhan
  • , Sebastian Jusuf
  • , Cheng Zong
  • , Mohamed N. Seleem
  • , George Y. Liu
  • , Qiang Cui
  • , Ji Xin Cheng
  • Boston University
  • Jilin University
  • University of California at San Diego
  • University of Pennsylvania
  • Purdue University
  • Rady Children's Hospital

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The rapid evolution of antibiotic resistance increasingly challenges the successful treatment of S. aureus infections. Here, we present an unconventional treatment approach by disassembly its membrane microdomains via pulsed laser photolysis of staphyloxanthin. After staphyloxanthin photolysis, membrane permeabilization, fluidification, and membrane protein detachment, were found the underlying mechanisms to malfunction its defense to several major classes of conventional antibiotics. Through resistance selection study, we found pulsed laser treatment completely depleted staphyloxanthin virulence. More importantly, laser treatment further inhibited development of resistance for several major classes of conventional antibiotics including fluoroquinolones, tetracyclines, aminoglycosides, and oxazolidinones. Collectively, this work highlights a novel platform to revive conventional antibiotics to treat S. aureus infections.

Original languageEnglish
Title of host publicationPhotonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020
EditorsTianhong Dai, Jurgen Popp, Mei X. Wu
PublisherSPIE
ISBN (Electronic)9781510632097
DOIs
StatePublished - 2020
EventPhotonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020 - San Francisco, United States
Duration: Feb 3 2020Feb 4 2020

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11223
ISSN (Print)1605-7422

Conference

ConferencePhotonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020
Country/TerritoryUnited States
CitySan Francisco
Period02/3/2002/4/20

Keywords

  • Antibiotic resistance
  • Membrane microdomain
  • MRSA
  • Photolysis
  • Phototherapy
  • Pulsed laser
  • S. aureus
  • Staphyloxanthin

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