Isolation of a cDNA clone for rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase

Ann D. Colosia; Mark Lively; M. Raafat El-Maghrabi; Simon J. Pilkis

doi:10.1016/0006-291X(87)90364-0

Isolation of a cDNA clone for rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase

Ann D. Colosia
, Mark Lively
, M. Raafat El-Maghrabi
, Simon J. Pilkis

Physiology and Biophysics

Vanderbilt University
Wake Forest University
Stony Brook University

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

A cDNA clone for rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase was isolated from a λgtll rat liver expression library by antibody screening. The clone was approximately 1100 bases in length and the derived amino acid sequence contained 303 residues at the carboxyl end of the subunit. This derived amino acid sequence corresponded exactly with the actual amino acid sequence of the enzyme determined by direct sequencing of the protein.

Original language	English
Pages (from-to)	1092-1098
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	143
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(87)90364-0
State	Published - Mar 30 1987

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title = "Isolation of a cDNA clone for rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase",

abstract = "A cDNA clone for rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase was isolated from a λgtll rat liver expression library by antibody screening. The clone was approximately 1100 bases in length and the derived amino acid sequence contained 303 residues at the carboxyl end of the subunit. This derived amino acid sequence corresponded exactly with the actual amino acid sequence of the enzyme determined by direct sequencing of the protein.",

author = "Colosia, \{Ann D.\} and Mark Lively and El-Maghrabi, \{M. Raafat\} and Pilkis, \{Simon J.\}",

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AU - Colosia, Ann D.

AU - Lively, Mark

AU - El-Maghrabi, M. Raafat

AU - Pilkis, Simon J.

PY - 1987/3/30

Y1 - 1987/3/30

N2 - A cDNA clone for rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase was isolated from a λgtll rat liver expression library by antibody screening. The clone was approximately 1100 bases in length and the derived amino acid sequence contained 303 residues at the carboxyl end of the subunit. This derived amino acid sequence corresponded exactly with the actual amino acid sequence of the enzyme determined by direct sequencing of the protein.

AB - A cDNA clone for rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase was isolated from a λgtll rat liver expression library by antibody screening. The clone was approximately 1100 bases in length and the derived amino acid sequence contained 303 residues at the carboxyl end of the subunit. This derived amino acid sequence corresponded exactly with the actual amino acid sequence of the enzyme determined by direct sequencing of the protein.

UR - https://www.scopus.com/pages/publications/0023117438

U2 - 10.1016/0006-291X(87)90364-0

DO - 10.1016/0006-291X(87)90364-0

M3 - Article

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AN - SCOPUS:0023117438

SN - 0006-291X

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JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Isolation of a cDNA clone for rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase

Abstract

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