Isolation of Native DNA Fragments Containing Structural Genes At The Beginning, in The Middle Or At The End of the Coding Strand

G. N. Yenikolopov; A. P. Ryskov; T. Nitta; G. P. Georgiev

doi:10.1093/nar/3.10.2645

Isolation of Native DNA Fragments Containing Structural Genes At The Beginning, in The Middle Or At The End of the Coding Strand

G. N. Yenikolopov
, A. P. Ryskov
, T. Nitta
, G. P. Georgiev

Anesthesiology

Academy of Sciences of the U.S.S.R.

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A method for the isolation of the DNA fragments containing structural genes at the beginning, in the middle and at the end of the coding strand is described. It involves mechanical or enzymatic shearing, exonuclease treatment, hybridization with mRNA and subsequent retention of DNA-mRNA complex on poly(U)-Sepharose. Hybridization of the DNA fragments with mRNA takes place with high specificity and isolated material is enriched in structural genes. Applications of this method for gene isolation are discussed.

Original language	English
Pages (from-to)	2645-2664
Number of pages	20
Journal	Nucleic Acids Research
Volume	3
Issue number	10
DOIs	https://doi.org/10.1093/nar/3.10.2645
State	Published - Oct 1976

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abstract = "A method for the isolation of the DNA fragments containing structural genes at the beginning, in the middle and at the end of the coding strand is described. It involves mechanical or enzymatic shearing, exonuclease treatment, hybridization with mRNA and subsequent retention of DNA-mRNA complex on poly(U)-Sepharose. Hybridization of the DNA fragments with mRNA takes place with high specificity and isolated material is enriched in structural genes. Applications of this method for gene isolation are discussed.",

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AU - Nitta, T.

AU - Georgiev, G. P.

PY - 1976/10

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N2 - A method for the isolation of the DNA fragments containing structural genes at the beginning, in the middle and at the end of the coding strand is described. It involves mechanical or enzymatic shearing, exonuclease treatment, hybridization with mRNA and subsequent retention of DNA-mRNA complex on poly(U)-Sepharose. Hybridization of the DNA fragments with mRNA takes place with high specificity and isolated material is enriched in structural genes. Applications of this method for gene isolation are discussed.

AB - A method for the isolation of the DNA fragments containing structural genes at the beginning, in the middle and at the end of the coding strand is described. It involves mechanical or enzymatic shearing, exonuclease treatment, hybridization with mRNA and subsequent retention of DNA-mRNA complex on poly(U)-Sepharose. Hybridization of the DNA fragments with mRNA takes place with high specificity and isolated material is enriched in structural genes. Applications of this method for gene isolation are discussed.

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Isolation of Native DNA Fragments Containing Structural Genes At The Beginning, in The Middle Or At The End of the Coding Strand

Abstract

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