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Laser capture microdissection of kidney tissue

  • Icahn School of Medicine at Mount Sinai

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

9 Scopus citations

Abstract

Kidney tissue laser capture microdissection (LCM) is of great clinical relevance since genome wide studies on total kidney messenger RNA (mRNA) potentially miss important factors involved in the pathogenesis of the disease in glomeruli and tubules. This technique is readily applicable to study mRNA from isolated glomeruli and tubules of human kidney biopsy material. In this chapter we present a "cook-book" practical approach of utilizing LCM in combination with RNA isolation technique in downstream applications in nephrology.

Original languageEnglish
Title of host publicationKidney Research
Subtitle of host publicationExperimental Protocols
PublisherHumana Press
Pages73-82
Number of pages10
ISBN (Print)9781588299451
DOIs
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
Volume466
ISSN (Print)1064-3745

Keywords

  • Glomerular isolation
  • RNA quality and quantity
  • Tubular dissection

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