Light as a substitute for glutaminic acid oxidase and fluorimetric determination of glutaminic acid

Weiping Cai; Xisheng Wang; Qingzhi Zhu; Zhihong Wang; Jingou Xu

Light as a substitute for glutaminic acid oxidase and fluorimetric determination of glutaminic acid

Weiping Cai
, Xisheng Wang
, Qingzhi Zhu
, Zhihong Wang
, Jingou Xu

Office of the Dean SOMAS

Xiamen University

Research output: Contribution to journal › Article › peer-review

Abstract

Under the irradiation of UV-light, photochemical reaction of glutaminic acid occur and produces H₂O₂ and NH₃ in weak alkaline medium with acetone. This reaction is equal in value for enzymatic reaction of glutaminic acid in the presence of glutaminic acid oxidase. Then, using hemin as catalyst, H₂O₂ produced by photochemical reaction oxidazes p-hydroxyphenyl acetic acid (HPA) and causes fluorescence reaction coupled of it. Based on this result, a new fluorimetric method was developed for indirect determination of glutaminic acid.

Original language	English
Pages (from-to)	1286
Number of pages	1
Journal	Fenxi Huaxue
Volume	28
Issue number	10
State	Published - 2000

Keywords

Fluorimetry
Glutaminic acid
Photochemical reaction
Substitution for enzyme

Cite this

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title = "Light as a substitute for glutaminic acid oxidase and fluorimetric determination of glutaminic acid",

abstract = "Under the irradiation of UV-light, photochemical reaction of glutaminic acid occur and produces H2O2 and NH3 in weak alkaline medium with acetone. This reaction is equal in value for enzymatic reaction of glutaminic acid in the presence of glutaminic acid oxidase. Then, using hemin as catalyst, H2O2 produced by photochemical reaction oxidazes p-hydroxyphenyl acetic acid (HPA) and causes fluorescence reaction coupled of it. Based on this result, a new fluorimetric method was developed for indirect determination of glutaminic acid.",

keywords = "Fluorimetry, Glutaminic acid, Photochemical reaction, Substitution for enzyme",

author = "Weiping Cai and Xisheng Wang and Qingzhi Zhu and Zhihong Wang and Jingou Xu",

year = "2000",

language = "English",

volume = "28",

pages = "1286",

journal = "Fenxi Huaxue",

issn = "0253-3820",

number = "10",

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TY - JOUR

T1 - Light as a substitute for glutaminic acid oxidase and fluorimetric determination of glutaminic acid

AU - Cai, Weiping

AU - Wang, Xisheng

AU - Zhu, Qingzhi

AU - Wang, Zhihong

AU - Xu, Jingou

PY - 2000

Y1 - 2000

N2 - Under the irradiation of UV-light, photochemical reaction of glutaminic acid occur and produces H2O2 and NH3 in weak alkaline medium with acetone. This reaction is equal in value for enzymatic reaction of glutaminic acid in the presence of glutaminic acid oxidase. Then, using hemin as catalyst, H2O2 produced by photochemical reaction oxidazes p-hydroxyphenyl acetic acid (HPA) and causes fluorescence reaction coupled of it. Based on this result, a new fluorimetric method was developed for indirect determination of glutaminic acid.

AB - Under the irradiation of UV-light, photochemical reaction of glutaminic acid occur and produces H2O2 and NH3 in weak alkaline medium with acetone. This reaction is equal in value for enzymatic reaction of glutaminic acid in the presence of glutaminic acid oxidase. Then, using hemin as catalyst, H2O2 produced by photochemical reaction oxidazes p-hydroxyphenyl acetic acid (HPA) and causes fluorescence reaction coupled of it. Based on this result, a new fluorimetric method was developed for indirect determination of glutaminic acid.

KW - Fluorimetry

KW - Glutaminic acid

KW - Photochemical reaction

KW - Substitution for enzyme

UR - https://www.scopus.com/pages/publications/9644272815

M3 - Article

AN - SCOPUS:9644272815

SN - 0253-3820

VL - 28

SP - 1286

JO - Fenxi Huaxue

JF - Fenxi Huaxue

IS - 10

ER -

Light as a substitute for glutaminic acid oxidase and fluorimetric determination of glutaminic acid

Abstract

Keywords

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