Limulus ventral photoreceptors contain a homologue of the α-subunit of mammalian Ns

Craig C. Malbon; U. B. Kaupp; J. E. Brown

doi:10.1016/0014-5793(84)80880-7

Limulus ventral photoreceptors contain a homologue of the α-subunit of mammalian N_s

Craig C. Malbon
, U. B. Kaupp
, J. E. Brown

Pharmacological Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Membranes from ventral photoreceptors of Limuluswere incubated with cholera toxin and [³²P]NAD⁺. Cholera toxin catalyzes a specific ADP-ribosylation of a 43-kDa peptide from Limulus ventral photoreceptors. Possible homologies between the 43-kDa peptide of Limulus and the α-subunits of mammalian stimulatory, guanine nucleotide-binding regulatory component of adenylate cyclase (N_s) were investigated by comparing the electrophoretic patterns of proteolytic fragments derived from each of these peptides that are radiolabeled by [³²P]NAD⁺ and cholera toxin. Evidence is provided for structural homology between this invertebrate peptide and mammalian N_s.

Original language	English
Pages (from-to)	91-94
Number of pages	4
Journal	FEBS Letters
Volume	172
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(84)80880-7
State	Published - Jun 25 1984

Keywords

Limulus Photoreceptor Cholera toxin ADP-ribosylation Stimulatory guanine nucleotide-binding regulatory component

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10.1016/0014-5793(84)80880-7

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title = "Limulus ventral photoreceptors contain a homologue of the α-subunit of mammalian Ns",

abstract = "Membranes from ventral photoreceptors of Limuluswere incubated with cholera toxin and [32P]NAD+. Cholera toxin catalyzes a specific ADP-ribosylation of a 43-kDa peptide from Limulus ventral photoreceptors. Possible homologies between the 43-kDa peptide of Limulus and the α-subunits of mammalian stimulatory, guanine nucleotide-binding regulatory component of adenylate cyclase (Ns) were investigated by comparing the electrophoretic patterns of proteolytic fragments derived from each of these peptides that are radiolabeled by [32P]NAD+ and cholera toxin. Evidence is provided for structural homology between this invertebrate peptide and mammalian Ns.",

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year = "1984",

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T1 - Limulus ventral photoreceptors contain a homologue of the α-subunit of mammalian Ns

AU - Malbon, Craig C.

AU - Kaupp, U. B.

AU - Brown, J. E.

PY - 1984/6/25

Y1 - 1984/6/25

N2 - Membranes from ventral photoreceptors of Limuluswere incubated with cholera toxin and [32P]NAD+. Cholera toxin catalyzes a specific ADP-ribosylation of a 43-kDa peptide from Limulus ventral photoreceptors. Possible homologies between the 43-kDa peptide of Limulus and the α-subunits of mammalian stimulatory, guanine nucleotide-binding regulatory component of adenylate cyclase (Ns) were investigated by comparing the electrophoretic patterns of proteolytic fragments derived from each of these peptides that are radiolabeled by [32P]NAD+ and cholera toxin. Evidence is provided for structural homology between this invertebrate peptide and mammalian Ns.

AB - Membranes from ventral photoreceptors of Limuluswere incubated with cholera toxin and [32P]NAD+. Cholera toxin catalyzes a specific ADP-ribosylation of a 43-kDa peptide from Limulus ventral photoreceptors. Possible homologies between the 43-kDa peptide of Limulus and the α-subunits of mammalian stimulatory, guanine nucleotide-binding regulatory component of adenylate cyclase (Ns) were investigated by comparing the electrophoretic patterns of proteolytic fragments derived from each of these peptides that are radiolabeled by [32P]NAD+ and cholera toxin. Evidence is provided for structural homology between this invertebrate peptide and mammalian Ns.

KW - Limulus Photoreceptor Cholera toxin ADP-ribosylation Stimulatory guanine nucleotide-binding regulatory component

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AN - SCOPUS:0021770631

SN - 0014-5793

VL - 172

SP - 91

EP - 94

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Limulus ventral photoreceptors contain a homologue of the α-subunit of mammalian N_s

Abstract

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