Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219

Melanie J. Dobson; A. Bruce Futcher; Brian S. Cox

doi:10.1007/BF00435686

Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219

Melanie J. Dobson
, A. Bruce Futcher
, Brian S. Cox

Microbiology and Immunology

University of Oxford

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Two plasmids containing Saccharomyces cerevisiae 2 μm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 μm DNA; in the presence of the LEU2 plasmids, 2 μm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 μm DNA loss has occurred, so that strains completely lacking 2 μm DNA sequences can be obtained routinely.

Original language	English
Pages (from-to)	201-205
Number of pages	5
Journal	Current Genetics
Volume	2
Issue number	3
DOIs	https://doi.org/10.1007/BF00435686
State	Published - Dec 1980

Keywords

2 μm DNA loss
Chimaeric plasmid instability

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10.1007/BF00435686

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title = "Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219",

abstract = "Two plasmids containing Saccharomyces cerevisiae 2 μm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 μm DNA; in the presence of the LEU2 plasmids, 2 μm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 μm DNA loss has occurred, so that strains completely lacking 2 μm DNA sequences can be obtained routinely.",

keywords = "2 μm DNA loss, Chimaeric plasmid instability",

author = "Dobson, \{Melanie J.\} and Futcher, \{A. Bruce\} and Cox, \{Brian S.\}",

year = "1980",

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doi = "10.1007/BF00435686",

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AU - Dobson, Melanie J.

AU - Futcher, A. Bruce

AU - Cox, Brian S.

PY - 1980/12

Y1 - 1980/12

N2 - Two plasmids containing Saccharomyces cerevisiae 2 μm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 μm DNA; in the presence of the LEU2 plasmids, 2 μm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 μm DNA loss has occurred, so that strains completely lacking 2 μm DNA sequences can be obtained routinely.

AB - Two plasmids containing Saccharomyces cerevisiae 2 μm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 μm DNA; in the presence of the LEU2 plasmids, 2 μm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 μm DNA loss has occurred, so that strains completely lacking 2 μm DNA sequences can be obtained routinely.

KW - 2 μm DNA loss

KW - Chimaeric plasmid instability

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U2 - 10.1007/BF00435686

DO - 10.1007/BF00435686

M3 - Article

AN - SCOPUS:0009165682

SN - 0172-8083

VL - 2

SP - 201

EP - 205

JO - Current Genetics

JF - Current Genetics

IS - 3

ER -

Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219

Abstract

Keywords

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