Low-frequency EPR study of chromium(V) formation from chromium(VI) in living plants

Ke Jian Liu; Jinjie Jiang; Xianglin Shi; Halina Gabrys; Tadeusz Walczak; Harold M. Swartz

doi:10.1006/bbrc.1995.1118

Low-frequency EPR study of chromium(V) formation from chromium(VI) in living plants

Ke Jian Liu
, Jinjie Jiang
, Xianglin Shi
, Halina Gabrys
, Tadeusz Walczak
, Harold M. Swartz

Pathology

Dartmouth College
National Institutes of Health
Jagiellonian University in Kraków

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

The reduction of Cr(VI) by green algae and higher plants was investigated using a low-frequency EPR spectrometer equipped with an extended loop gap resonator. Incubation of algae (Spirogyra and Mongeotia) with Cr(VI) generated both Cr(V) and Cr(III). The maximum Cr(V) signal was observed in about 10 minutes. Incubation of Cr(VI) with oat, soybean, and garlic generated Cr(V). The maximum Cr(V) peak appeared after more than 10 hours of incubation, and Cr(V) was located predominantly in the roots. The Cr(V) peak exhibited hyperfine splittings of about 0.79 gauss, typical of the Cr(V) complexes with diol-containing molecules. The results suggest that the reduction of Cr(VI) to lower oxidation states by living plants may provide a detoxification pathway for Cr(VI) in ecological systems. The results also indicate that low-frequency EPR may be used to investigate the metabolism of paramagnetic metal ions in intact plants.

Original language	English
Pages (from-to)	829-834
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	206
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1995.1118
State	Published - 1995

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@article{ba37ee4972004446aa3e653051c519ff,

title = "Low-frequency EPR study of chromium(V) formation from chromium(VI) in living plants",

abstract = "The reduction of Cr(VI) by green algae and higher plants was investigated using a low-frequency EPR spectrometer equipped with an extended loop gap resonator. Incubation of algae (Spirogyra and Mongeotia) with Cr(VI) generated both Cr(V) and Cr(III). The maximum Cr(V) signal was observed in about 10 minutes. Incubation of Cr(VI) with oat, soybean, and garlic generated Cr(V). The maximum Cr(V) peak appeared after more than 10 hours of incubation, and Cr(V) was located predominantly in the roots. The Cr(V) peak exhibited hyperfine splittings of about 0.79 gauss, typical of the Cr(V) complexes with diol-containing molecules. The results suggest that the reduction of Cr(VI) to lower oxidation states by living plants may provide a detoxification pathway for Cr(VI) in ecological systems. The results also indicate that low-frequency EPR may be used to investigate the metabolism of paramagnetic metal ions in intact plants.",

author = "Liu, \{Ke Jian\} and Jinjie Jiang and Xianglin Shi and Halina Gabrys and Tadeusz Walczak and Swartz, \{Harold M.\}",

year = "1995",

doi = "10.1006/bbrc.1995.1118",

language = "English",

volume = "206",

pages = "829--834",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

number = "3",

}

TY - JOUR

T1 - Low-frequency EPR study of chromium(V) formation from chromium(VI) in living plants

AU - Liu, Ke Jian

AU - Jiang, Jinjie

AU - Shi, Xianglin

AU - Gabrys, Halina

AU - Walczak, Tadeusz

AU - Swartz, Harold M.

PY - 1995

Y1 - 1995

N2 - The reduction of Cr(VI) by green algae and higher plants was investigated using a low-frequency EPR spectrometer equipped with an extended loop gap resonator. Incubation of algae (Spirogyra and Mongeotia) with Cr(VI) generated both Cr(V) and Cr(III). The maximum Cr(V) signal was observed in about 10 minutes. Incubation of Cr(VI) with oat, soybean, and garlic generated Cr(V). The maximum Cr(V) peak appeared after more than 10 hours of incubation, and Cr(V) was located predominantly in the roots. The Cr(V) peak exhibited hyperfine splittings of about 0.79 gauss, typical of the Cr(V) complexes with diol-containing molecules. The results suggest that the reduction of Cr(VI) to lower oxidation states by living plants may provide a detoxification pathway for Cr(VI) in ecological systems. The results also indicate that low-frequency EPR may be used to investigate the metabolism of paramagnetic metal ions in intact plants.

AB - The reduction of Cr(VI) by green algae and higher plants was investigated using a low-frequency EPR spectrometer equipped with an extended loop gap resonator. Incubation of algae (Spirogyra and Mongeotia) with Cr(VI) generated both Cr(V) and Cr(III). The maximum Cr(V) signal was observed in about 10 minutes. Incubation of Cr(VI) with oat, soybean, and garlic generated Cr(V). The maximum Cr(V) peak appeared after more than 10 hours of incubation, and Cr(V) was located predominantly in the roots. The Cr(V) peak exhibited hyperfine splittings of about 0.79 gauss, typical of the Cr(V) complexes with diol-containing molecules. The results suggest that the reduction of Cr(VI) to lower oxidation states by living plants may provide a detoxification pathway for Cr(VI) in ecological systems. The results also indicate that low-frequency EPR may be used to investigate the metabolism of paramagnetic metal ions in intact plants.

UR - https://www.scopus.com/pages/publications/0028895571

U2 - 10.1006/bbrc.1995.1118

DO - 10.1006/bbrc.1995.1118

M3 - Article

AN - SCOPUS:0028895571

SN - 0006-291X

VL - 206

SP - 829

EP - 834

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Low-frequency EPR study of chromium(V) formation from chromium(VI) in living plants

Abstract

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