@inbook{222c2102c8334f06bf9542deefc4d769,
title = "Measuring Replicative Lifespan in Cryptococcus neoformans",
abstract = "Advances in understanding cellular aging research have been possible due to the analysis of the replicative lifespan of yeast cells. Studying longevity in the pathogenic yeast Cryptococcus neoformans is essential because old yeast cells with age-related phenotypes accumulate during infection and are associated with increased virulence and antifungal tolerance. Microdissection and microfluidic devices are valuable tools for continuously tracking cells at the single-cell level. In this chapter, we describe the features of these two platforms and outline technical limitations and information to study aging mechanisms while assessing the lifespan of yeast cells.",
keywords = "Aging, Longevity, Microdissection, Microfluidic system, Yeast",
author = "Silva, \{Vanessa K.A.\} and Oliveira, \{Natalia K.\} and Fries, \{Bettina C.\}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3722-7\_25",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "375--384",
booktitle = "Methods in Molecular Biology",
}