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Mesoscopic Fluorescence Imaging of Light Triggered Chemotherapeutic Release in a Cancer Spheroid Model

  • Elias Kluiszo
  • , Rasel Ahmmed
  • , Berna Aliu
  • , Semra Aygun-Sunar
  • , Luigi Belcastro
  • , Matthew Willadsen
  • , Hilliard L. Kutscher
  • , Jonathan F. Lovell
  • , Ulas Sunar
  • Stony Brook University
  • POP Biotechnologies
  • SUNY Buffalo

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Peritoneal micrometastases (micromets) represent a significant clinical challenge across a wide spectrum of malignancies due to their aggressive progression and limited treatment efficacy. Liposomal Doxorubicin (Dox) is a viable drug formulation that improves the biodistribution and availability of the Dox chemotherapeutic, but is hindered by reduced release kinetics. Recent advances in this formulation enable light-triggered release of Dox from the liposome, resulting in targeted chemotherapy with reduced effects on the surrounding tissue. Light-triggered release of Dox at a tumor site functions in both a therapeutic and diagnostic capacity, as its inherent fluorescence allows for analysis of disease progression and can provide contrast for fluorescence-guided surgery. Here we examine light-triggered Dox as a contrast method for fluorescence-guided surgery by culturing the drug within a cancer spheroid model and imaging fluorescence with a laparoscopic system. Results from this method show promise in the use of Dox as a contrast marker for fluorescence-guided surgery in an imaging modality suitable for minimally-invasive micromet resection.

Original languageEnglish
Title of host publicationMolecular-Guided Surgery
Subtitle of host publicationMolecules, Devices, and Applications XII
EditorsSummer L. Gibbs, Kenneth M. Tichauer
PublisherSPIE
ISBN (Electronic)9781510695771
DOIs
StatePublished - Mar 5 2026
Event12th Molecular-Guided Surgery: Molecules, Devices, and Applications - San Francisco, United States
Duration: Jan 17 2026Jan 20 2026

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume13832
ISSN (Print)1605-7422
ISSN (Electronic)2410-9045

Conference

Conference12th Molecular-Guided Surgery: Molecules, Devices, and Applications
Country/TerritoryUnited States
CitySan Francisco
Period01/17/2601/20/26

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