TY - CHAP
T1 - Metabolic Labeling of Glycans with Azido Sugars for Visualization and Glycoproteomics
AU - Laughlin, Scott T.
AU - Agard, Nicholas J.
AU - Baskin, Jeremy M.
AU - Carrico, Isaac S.
AU - Chang, Pamela V.
AU - Ganguli, Anjali S.
AU - Hangauer, Matthew J.
AU - Lo, Anderson
AU - Prescher, Jennifer A.
AU - Bertozzi, Carolyn R.
PY - 2006
Y1 - 2006
N2 - The staggering complexity of glycans renders their analysis extraordinarily difficult, particularly in living systems. A recently developed technology, termed metabolic oligosaccharide engineering, enables glycan labeling with probes for visualization in cells and living animals, and enrichment of specific glycoconjugate types for proteomic analysis. This technology involves metabolic labeling of glycans with a specifically reactive, abiotic functional group, the azide. Azido sugars are fed to cells and integrated by the glycan biosynthetic machinery into various glycoconjugates. The azido sugars are then covalently tagged, either ex vivo or in vivo, using one of two azide-specific chemistries: the Staudinger ligation, or the strain-promoted [3+2] cycloaddition. These reactions can be used to tag glycans with imaging probes or epitope tags, thus enabling the visualization or enrichment of glycoconjugates. Applications to noninvasive imaging and glycoproteomic analyses are discussed.
AB - The staggering complexity of glycans renders their analysis extraordinarily difficult, particularly in living systems. A recently developed technology, termed metabolic oligosaccharide engineering, enables glycan labeling with probes for visualization in cells and living animals, and enrichment of specific glycoconjugate types for proteomic analysis. This technology involves metabolic labeling of glycans with a specifically reactive, abiotic functional group, the azide. Azido sugars are fed to cells and integrated by the glycan biosynthetic machinery into various glycoconjugates. The azido sugars are then covalently tagged, either ex vivo or in vivo, using one of two azide-specific chemistries: the Staudinger ligation, or the strain-promoted [3+2] cycloaddition. These reactions can be used to tag glycans with imaging probes or epitope tags, thus enabling the visualization or enrichment of glycoconjugates. Applications to noninvasive imaging and glycoproteomic analyses are discussed.
UR - https://www.scopus.com/pages/publications/33750995248
U2 - 10.1016/S0076-6879(06)15015-6
DO - 10.1016/S0076-6879(06)15015-6
M3 - Chapter
C2 - 17116478
AN - SCOPUS:33750995248
SN - 0121828204
SN - 9780121828202
T3 - Methods in Enzymology
SP - 230
EP - 250
BT - Glycobiology
PB - Academic Press Inc.
ER -