TY - GEN
T1 - Micro-stamped ECM proteins enhance endothelial cell adhesion and directed growth
AU - Rubenstein, D. A.
AU - Frame, M. D.
PY - 2007
Y1 - 2007
N2 - Our goal was to evaluate microvascular endothelial cell growth on hydrophilic glass surfaces micro-stamped with extracellular matrix proteins (ECM). A combination of photo- and soft-lithography was used to fabricate microstamps. We hypothesized that by day 3, human dermal microvascular endothelial cells (hDMECs) would have higher viability and density on the patterned matrices vs. on bare glass. Also, our preferential elongated morphology would be heightened on the stamped matrix. By day 3, we saw a four-fold increase in density on the stamped proteins. Viability was independent of the ECM type (fibronectin, laminin, collagen I and IV). Endothelial cell morphology was preferentially elongated on each ECM type, more so than on bare glass. This demonstrates the ability to concentrate the growth of endothelial cells within regions of microstamped ECM proteins.
AB - Our goal was to evaluate microvascular endothelial cell growth on hydrophilic glass surfaces micro-stamped with extracellular matrix proteins (ECM). A combination of photo- and soft-lithography was used to fabricate microstamps. We hypothesized that by day 3, human dermal microvascular endothelial cells (hDMECs) would have higher viability and density on the patterned matrices vs. on bare glass. Also, our preferential elongated morphology would be heightened on the stamped matrix. By day 3, we saw a four-fold increase in density on the stamped proteins. Viability was independent of the ECM type (fibronectin, laminin, collagen I and IV). Endothelial cell morphology was preferentially elongated on each ECM type, more so than on bare glass. This demonstrates the ability to concentrate the growth of endothelial cells within regions of microstamped ECM proteins.
UR - https://www.scopus.com/pages/publications/48749121790
U2 - 10.1109/NEBC.2007.4413339
DO - 10.1109/NEBC.2007.4413339
M3 - Conference contribution
AN - SCOPUS:48749121790
SN - 1424410339
SN - 9781424410330
T3 - Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
SP - 183
EP - 184
BT - 33rd Annual Northeast Bioengineering Conference - Engineering Innovations in Life Sciences and Healthcare, NEBC
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 33rd Annual Northeast Bioengineering Conference, NEBC
Y2 - 10 March 2007 through 11 March 2007
ER -