TY - GEN
T1 - Microvascular network shear rate is disrupted with elevated tissue bath glucose in normal mice
AU - Georgi, M. K.B.
AU - Sharma, S.
AU - Frame, M. D.
PY - 2007
Y1 - 2007
N2 - Blood glucose levels reflect interstitial glucose in both normal and diabetics patients. Long term elevated blood glucose adversely affects shear sensing in the microcirculaton1. We have shown that there is a predictable axial gradient in shear within arteriolar networks2. We hypothesized that elevated interstitial glucose levels in non-diabetic mice would disrupt axial gradients in shear rate (SR). Networks were observed in the cremaster muscle of anesthetized (n=6) C57BL/6 mice before and after 2 hours exposure to tissue bath glucose (Glc). Diameter (D) and red blood cell (RBC) velocity (V) were measured and SR rate was calculated (8V/D). In controls, SR was lower for the feed compared to associated branches, with SR in the upstream branches higher and more variable than in the last branch of the network. With Glc SR decreased in the feed; all cell flux was diverted from upstream branches to the last branch of the network. Feed diameter dilated, but branch diameter was unaffected by glucose; thus changes in SR were not attributed to changes in resistance alone. We conclude that only 2 hours of high glucose adversely affected flow distribution and axial shear gradients within this muscle preparation.
AB - Blood glucose levels reflect interstitial glucose in both normal and diabetics patients. Long term elevated blood glucose adversely affects shear sensing in the microcirculaton1. We have shown that there is a predictable axial gradient in shear within arteriolar networks2. We hypothesized that elevated interstitial glucose levels in non-diabetic mice would disrupt axial gradients in shear rate (SR). Networks were observed in the cremaster muscle of anesthetized (n=6) C57BL/6 mice before and after 2 hours exposure to tissue bath glucose (Glc). Diameter (D) and red blood cell (RBC) velocity (V) were measured and SR rate was calculated (8V/D). In controls, SR was lower for the feed compared to associated branches, with SR in the upstream branches higher and more variable than in the last branch of the network. With Glc SR decreased in the feed; all cell flux was diverted from upstream branches to the last branch of the network. Feed diameter dilated, but branch diameter was unaffected by glucose; thus changes in SR were not attributed to changes in resistance alone. We conclude that only 2 hours of high glucose adversely affected flow distribution and axial shear gradients within this muscle preparation.
UR - https://www.scopus.com/pages/publications/48749117502
U2 - 10.1109/NEBC.2007.4413333
DO - 10.1109/NEBC.2007.4413333
M3 - Conference contribution
AN - SCOPUS:48749117502
SN - 1424410339
SN - 9781424410330
T3 - Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
SP - 171
EP - 172
BT - 33rd Annual Northeast Bioengineering Conference - Engineering Innovations in Life Sciences and Healthcare, NEBC
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 33rd Annual Northeast Bioengineering Conference, NEBC
Y2 - 10 March 2007 through 11 March 2007
ER -