Mitigation of bone loss with ultrasound induced dynamic mechanical signals in an OVX induced rat model of osteopenia

Suzanne L. Ferreri; Roger Talish; Titi Trandafir; Yi Xian Qin

doi:10.1016/j.bone.2011.01.002

Mitigation of bone loss with ultrasound induced dynamic mechanical signals in an OVX induced rat model of osteopenia

Suzanne L. Ferreri
, Roger Talish
, Titi Trandafir
, Yi Xian Qin

Biomedical Engineering

Stony Brook University
Juvent, Inc.

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

This study tests the hypothesis that an ultrasound generated dynamic mechanical signal can attenuate bone loss in an estrogen deficient model of osteopenia. Eighty-four 16-week-old Sprague-Dawley rats were divided into six groups: baseline control, age-matched control, ovariectomy (OVX) control, OVX+5mW/cm² ultrasound (US), OVX+30mW/cm² US and OVX+100mW/cm² US. Low intensity pulsed ultrasound (LIPUS) was delivered transdermally at the L4/L5 vertebrae, using gel-coupled plane wave US transducers. The signal, characterized by 200μs pulses of 1.5MHz sine waves repeating at 1kHz with spatial-averaged temporal-averaged (SATA) intensities of 5, 30 or 100mW/cm², was applied 20min/day, 5days/week for 4weeks. OVX treatment reduced bone volume fraction 40% and compromised microstructure at 4weeks. LIPUS treatment, however, significantly increased BV/TV (+33%) compared to OVX controls for the 100mW/cm² treated group. SMI and Tb.N showed significant improvements compared with OVX for the 100mW/cm² treated group and Tb.Th was significantly improved in the 30 and 100mW/cm² treated groups. Improvements in bone's microstructural characteristics with 100mW/cm² US treatment translated into improved load bearing characteristics, including a significant 42% increase in apparent level elastic modulus compared to OVX controls. Significant improvement of trabecular mechanical strength was also observed in the treated animals, e.g., principal compressive stress (represent bone's ability to resist loads) was significantly higher compared to OVX controls. Histomorphometric analysis also showed that treatment with 100mW/cm² US resulted in a 76% improvement in MS/BS. In addition, measures of bone quantity and quality at the femoral metaphysis suggest that LIPUS is site specific. This study indicates that localized ultrasound treatment, delivered at specific intensities, has beneficial effects on intact bone and may represent a novel intervention for bone loss.

Original language	English
Pages (from-to)	1095-1102
Number of pages	8
Journal	Bone
Volume	48
Issue number	5
DOIs	https://doi.org/10.1016/j.bone.2011.01.002
State	Published - May 1 2011

Keywords

Bone remodeling
Low intensity ultrasound
Mechanotransduction
Ovariectomy
Therapeutic ultrasound

Access to Document

10.1016/j.bone.2011.01.002

Cite this

@article{d135bffcbdc943638bd95d6e28b66373,

title = "Mitigation of bone loss with ultrasound induced dynamic mechanical signals in an OVX induced rat model of osteopenia",

abstract = "This study tests the hypothesis that an ultrasound generated dynamic mechanical signal can attenuate bone loss in an estrogen deficient model of osteopenia. Eighty-four 16-week-old Sprague-Dawley rats were divided into six groups: baseline control, age-matched control, ovariectomy (OVX) control, OVX+5mW/cm2 ultrasound (US), OVX+30mW/cm2 US and OVX+100mW/cm2 US. Low intensity pulsed ultrasound (LIPUS) was delivered transdermally at the L4/L5 vertebrae, using gel-coupled plane wave US transducers. The signal, characterized by 200μs pulses of 1.5MHz sine waves repeating at 1kHz with spatial-averaged temporal-averaged (SATA) intensities of 5, 30 or 100mW/cm2, was applied 20min/day, 5days/week for 4weeks. OVX treatment reduced bone volume fraction 40\% and compromised microstructure at 4weeks. LIPUS treatment, however, significantly increased BV/TV (+33\%) compared to OVX controls for the 100mW/cm2 treated group. SMI and Tb.N showed significant improvements compared with OVX for the 100mW/cm2 treated group and Tb.Th was significantly improved in the 30 and 100mW/cm2 treated groups. Improvements in bone's microstructural characteristics with 100mW/cm2 US treatment translated into improved load bearing characteristics, including a significant 42\% increase in apparent level elastic modulus compared to OVX controls. Significant improvement of trabecular mechanical strength was also observed in the treated animals, e.g., principal compressive stress (represent bone's ability to resist loads) was significantly higher compared to OVX controls. Histomorphometric analysis also showed that treatment with 100mW/cm2 US resulted in a 76\% improvement in MS/BS. In addition, measures of bone quantity and quality at the femoral metaphysis suggest that LIPUS is site specific. This study indicates that localized ultrasound treatment, delivered at specific intensities, has beneficial effects on intact bone and may represent a novel intervention for bone loss.",

keywords = "Bone remodeling, Low intensity ultrasound, Mechanotransduction, Ovariectomy, Therapeutic ultrasound",

author = "Ferreri, \{Suzanne L.\} and Roger Talish and Titi Trandafir and Qin, \{Yi Xian\}",

year = "2011",

month = may,

day = "1",

doi = "10.1016/j.bone.2011.01.002",

language = "English",

volume = "48",

pages = "1095--1102",

journal = "Bone",

issn = "8756-3282",

number = "5",

}

TY - JOUR

T1 - Mitigation of bone loss with ultrasound induced dynamic mechanical signals in an OVX induced rat model of osteopenia

AU - Ferreri, Suzanne L.

AU - Talish, Roger

AU - Trandafir, Titi

AU - Qin, Yi Xian

PY - 2011/5/1

Y1 - 2011/5/1

N2 - This study tests the hypothesis that an ultrasound generated dynamic mechanical signal can attenuate bone loss in an estrogen deficient model of osteopenia. Eighty-four 16-week-old Sprague-Dawley rats were divided into six groups: baseline control, age-matched control, ovariectomy (OVX) control, OVX+5mW/cm2 ultrasound (US), OVX+30mW/cm2 US and OVX+100mW/cm2 US. Low intensity pulsed ultrasound (LIPUS) was delivered transdermally at the L4/L5 vertebrae, using gel-coupled plane wave US transducers. The signal, characterized by 200μs pulses of 1.5MHz sine waves repeating at 1kHz with spatial-averaged temporal-averaged (SATA) intensities of 5, 30 or 100mW/cm2, was applied 20min/day, 5days/week for 4weeks. OVX treatment reduced bone volume fraction 40% and compromised microstructure at 4weeks. LIPUS treatment, however, significantly increased BV/TV (+33%) compared to OVX controls for the 100mW/cm2 treated group. SMI and Tb.N showed significant improvements compared with OVX for the 100mW/cm2 treated group and Tb.Th was significantly improved in the 30 and 100mW/cm2 treated groups. Improvements in bone's microstructural characteristics with 100mW/cm2 US treatment translated into improved load bearing characteristics, including a significant 42% increase in apparent level elastic modulus compared to OVX controls. Significant improvement of trabecular mechanical strength was also observed in the treated animals, e.g., principal compressive stress (represent bone's ability to resist loads) was significantly higher compared to OVX controls. Histomorphometric analysis also showed that treatment with 100mW/cm2 US resulted in a 76% improvement in MS/BS. In addition, measures of bone quantity and quality at the femoral metaphysis suggest that LIPUS is site specific. This study indicates that localized ultrasound treatment, delivered at specific intensities, has beneficial effects on intact bone and may represent a novel intervention for bone loss.

AB - This study tests the hypothesis that an ultrasound generated dynamic mechanical signal can attenuate bone loss in an estrogen deficient model of osteopenia. Eighty-four 16-week-old Sprague-Dawley rats were divided into six groups: baseline control, age-matched control, ovariectomy (OVX) control, OVX+5mW/cm2 ultrasound (US), OVX+30mW/cm2 US and OVX+100mW/cm2 US. Low intensity pulsed ultrasound (LIPUS) was delivered transdermally at the L4/L5 vertebrae, using gel-coupled plane wave US transducers. The signal, characterized by 200μs pulses of 1.5MHz sine waves repeating at 1kHz with spatial-averaged temporal-averaged (SATA) intensities of 5, 30 or 100mW/cm2, was applied 20min/day, 5days/week for 4weeks. OVX treatment reduced bone volume fraction 40% and compromised microstructure at 4weeks. LIPUS treatment, however, significantly increased BV/TV (+33%) compared to OVX controls for the 100mW/cm2 treated group. SMI and Tb.N showed significant improvements compared with OVX for the 100mW/cm2 treated group and Tb.Th was significantly improved in the 30 and 100mW/cm2 treated groups. Improvements in bone's microstructural characteristics with 100mW/cm2 US treatment translated into improved load bearing characteristics, including a significant 42% increase in apparent level elastic modulus compared to OVX controls. Significant improvement of trabecular mechanical strength was also observed in the treated animals, e.g., principal compressive stress (represent bone's ability to resist loads) was significantly higher compared to OVX controls. Histomorphometric analysis also showed that treatment with 100mW/cm2 US resulted in a 76% improvement in MS/BS. In addition, measures of bone quantity and quality at the femoral metaphysis suggest that LIPUS is site specific. This study indicates that localized ultrasound treatment, delivered at specific intensities, has beneficial effects on intact bone and may represent a novel intervention for bone loss.

KW - Bone remodeling

KW - Low intensity ultrasound

KW - Mechanotransduction

KW - Ovariectomy

KW - Therapeutic ultrasound

UR - https://www.scopus.com/pages/publications/79954503362

U2 - 10.1016/j.bone.2011.01.002

DO - 10.1016/j.bone.2011.01.002

M3 - Article

C2 - 21241838

AN - SCOPUS:79954503362

SN - 8756-3282

VL - 48

SP - 1095

EP - 1102

JO - Bone

JF - Bone

IS - 5

ER -

Mitigation of bone loss with ultrasound induced dynamic mechanical signals in an OVX induced rat model of osteopenia

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this