TY - GEN
T1 - MULTICHANNEL IMPEDANCE CYTOMETRY DOWNSTREAM OF CELL SEPARATION FOR QUANTIFYING ENRICHMENT OF ACTIVATED MACROPHAGE SUBPOPULATIONS
AU - Jarmoshti, Javad
AU - Torres-Castro, Karina
AU - Xiao, Li
AU - Rane, Aditya
AU - Salahi, Armita
AU - Jin, Li
AU - Li, Xudong
AU - Caselli, Federica
AU - Honrado, Carlos
AU - Swami, Nathan
N1 - Publisher Copyright:
© 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Cell phenotype-specific microfluidic separation from heterogeneous samples is usually ascertained by imaging fluorescently labeled samples, which is not well suited for phenotypes lacking reliable cell surface markers and for samples utilized in transplant applications for regenerative medicine. The alternate validation method is through off-chip flow cytometry of the collected fractions after fluorescent staining, but this causes dilution of the enriched cell numbers for off-chip analysis. Instead, we present a method for on-chip label-free biophysical cytometry based on single-cell impedance cytometry downstream of cell separation. This is applied for on-chip quantification of the enrichment of activated macrophages by deterministic lateral displacement.
AB - Cell phenotype-specific microfluidic separation from heterogeneous samples is usually ascertained by imaging fluorescently labeled samples, which is not well suited for phenotypes lacking reliable cell surface markers and for samples utilized in transplant applications for regenerative medicine. The alternate validation method is through off-chip flow cytometry of the collected fractions after fluorescent staining, but this causes dilution of the enriched cell numbers for off-chip analysis. Instead, we present a method for on-chip label-free biophysical cytometry based on single-cell impedance cytometry downstream of cell separation. This is applied for on-chip quantification of the enrichment of activated macrophages by deterministic lateral displacement.
KW - Activation
KW - Deterministic Lateral Displacement
KW - Impedance Cytometry
KW - Macrophages
UR - https://www.scopus.com/pages/publications/85175692437
M3 - Conference contribution
AN - SCOPUS:85175692437
T3 - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 109
EP - 110
BT - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Y2 - 23 October 2022 through 27 October 2022
ER -