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Nanometer resolution hard X-ray microscopy of bone and mineralized tissue

  • M. E. Ruppel
  • , Y. Meng
  • , Y. Qin
  • , D. B. Burr
  • , M. R. Allen
  • , Y. F. Song
  • , L. M. Miller
  • Stony Brook University
  • Indiana University Bloomington
  • National Synchrotron Radiation Research Center Taiwan
  • Brookhaven National Laboratory

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Full field hard x-ray microscopy has recently been developed utilizing synchrotron light, enabling absorption and phase contrast imaging with a spatial resolution of 50 - 100 nm. This technique has allowed us to image bone tissue at the nanoscale level, viewing the substructure of mineralized collagen fibers. Thin sections of tissue (5 and 50 μm thick) from the L3 vertebrae of Beagles and mineralizing MC3T3-E1 mouse osteoblasts were imaged at beamline 01B1 at the National Synchrotron Radiation Research Center (Hsinchu, Taiwan). Collagen fiber orientation was observed in the lamellar structure of an osteon. Mineralizing osteoblasts were found to produce disordered mineralized nodules with varying density and structure.

Original languageEnglish
Title of host publication33rd Annual Northeast Bioengineering Conference - Engineering Innovations in Life Sciences and Healthcare, NEBC
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages33-34
Number of pages2
ISBN (Print)1424410339, 9781424410330
DOIs
StatePublished - 2007
Event33rd Annual Northeast Bioengineering Conference, NEBC - Stony Brook, NY, United States
Duration: Mar 10 2007Mar 11 2007

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
ISSN (Print)1071-121X

Conference

Conference33rd Annual Northeast Bioengineering Conference, NEBC
Country/TerritoryUnited States
CityStony Brook, NY
Period03/10/0703/11/07

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