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Nestin-based reporter transgenic mouse lines

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

13 Scopus citations

Abstract

Nestin expression marks stem and progenitor cells of the neural lineage. Transgenic mouse lines, originally generated to identify neural stem cells, can also help to identify, track, and isolate stem and progenitor cells in a range of tissues of the ectodermal, endodermal, and mesodermal origin. Here, we describe the generation of transgenic mouse lines expressing fluorescent proteins (FP) under the control of critical regulatory elements of the nestin gene and their use for identifying and analyzing adult stem and progenitor cells in various tissues.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages7-14
Number of pages8
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1453
ISSN (Print)1064-3745

Keywords

  • Hair-follicle-associated pluripotent (HAP)
  • Immunocytochemistry
  • Nestin
  • Nestin-GFP
  • Reporter lines
  • Stem cells
  • Transgenic animals

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