Nitric oxide synthase in the nervous system and ink gland of the cuttlefish Sepia officinalis: Molecular cloning and expression

Vladimir Scheinker; Gabriella Fiore; Carlo Di Cristo; Anna Di Cosmo; Marco D'Ischia; Grigori Enikolopov; Anna Palumbo

doi:10.1016/j.bbrc.2005.10.069

Nitric oxide synthase in the nervous system and ink gland of the cuttlefish Sepia officinalis: Molecular cloning and expression

Vladimir Scheinker
, Gabriella Fiore
, Carlo Di Cristo
, Anna Di Cosmo
, Marco D'Ischia
, Grigori Enikolopov
, Anna Palumbo

Anesthesiology

Cold Spring Harbor Laboratory
Stazione Zoologica Anton Dohrn Napoli
University of Sannio
University of Naples Federico II

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Nitric oxide (NO) signaling is involved in numerous physiological processes in mollusks, e.g., learning and memory, feeding behavior, neural development, and defence response. We report the first molecular cloning of NOS mRNA from a cephalopod, the cuttlefish Sepia officinalis (SoNOS). SoNOS was cloned using a strategy that involves hybridization of degenerate PCR primers to highly conserved NOS regions, combined with RACE procedure. Two splicing variants of SoNOS, differing by 18 nucleotides, were found in the nervous system and the ink gland of Sepia. In situ hybridization shows that SoNOS is expressed in the immature and mature cells of the ink gland and in the regions of the nervous system that are related to the ink defence system.

Original language	English
Pages (from-to)	1204-1215
Number of pages	12
Journal	Biochemical and Biophysical Research Communications
Volume	338
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2005.10.069
State	Published - Dec 16 2005

Keywords

Central nervous system
Ink gland
Molecular cloning
Nitric oxide synthase
Sepia officinalis
Splicing

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10.1016/j.bbrc.2005.10.069

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title = "Nitric oxide synthase in the nervous system and ink gland of the cuttlefish Sepia officinalis: Molecular cloning and expression",

abstract = "Nitric oxide (NO) signaling is involved in numerous physiological processes in mollusks, e.g., learning and memory, feeding behavior, neural development, and defence response. We report the first molecular cloning of NOS mRNA from a cephalopod, the cuttlefish Sepia officinalis (SoNOS). SoNOS was cloned using a strategy that involves hybridization of degenerate PCR primers to highly conserved NOS regions, combined with RACE procedure. Two splicing variants of SoNOS, differing by 18 nucleotides, were found in the nervous system and the ink gland of Sepia. In situ hybridization shows that SoNOS is expressed in the immature and mature cells of the ink gland and in the regions of the nervous system that are related to the ink defence system.",

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AU - Scheinker, Vladimir

AU - Fiore, Gabriella

AU - Di Cristo, Carlo

AU - Di Cosmo, Anna

AU - D'Ischia, Marco

AU - Enikolopov, Grigori

AU - Palumbo, Anna

PY - 2005/12/16

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AB - Nitric oxide (NO) signaling is involved in numerous physiological processes in mollusks, e.g., learning and memory, feeding behavior, neural development, and defence response. We report the first molecular cloning of NOS mRNA from a cephalopod, the cuttlefish Sepia officinalis (SoNOS). SoNOS was cloned using a strategy that involves hybridization of degenerate PCR primers to highly conserved NOS regions, combined with RACE procedure. Two splicing variants of SoNOS, differing by 18 nucleotides, were found in the nervous system and the ink gland of Sepia. In situ hybridization shows that SoNOS is expressed in the immature and mature cells of the ink gland and in the regions of the nervous system that are related to the ink defence system.

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KW - Ink gland

KW - Molecular cloning

KW - Nitric oxide synthase

KW - Sepia officinalis

KW - Splicing

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Nitric oxide synthase in the nervous system and ink gland of the cuttlefish Sepia officinalis: Molecular cloning and expression

Abstract

Keywords

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