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Noninvasive imaging of absolute PpIX concentration distribution in nonmelanoma skin tumors at pre-PDT

  • Ulas Sunar
  • , Daniel J. Rohrbach
  • , Janet Morgan
  • , Natalie Zeitouni
  • Roswell Park Cancer Institute

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Photodynamic Therapy (PDT) has proven to be an effective treatment option for nonmelanoma skin cancers. The ability to quantify the concentration of drug in the treated area is crucial for effective treatment planning as well as predicting outcomes. We utilized spatial frequency domain imaging for quantifying the accurate concentration of protoporphyrin IX (PpIX) in phantoms and in vivo. We correct fluorescence against the effects of native tissue absorption and scattering parameters. First we quantified the absorption and scattering of the tissue non-invasively. Then, we corrected raw fluorescence signal by compensating for optical properties to get the absolute drug concentration. After phantom experiments, we used basal cell carcinoma (BCC) model in Gli mice to determine optical properties and drug concentration in vivo at pre-PDT.

Original languageEnglish
Title of host publicationOptical Methods for Tumor Treatment and Detection
Subtitle of host publicationMechanisms and Techniques in Photodynamic Therapy XXII
DOIs
StatePublished - 2013
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII - San Francisco, CA, United States
Duration: Feb 2 2013Feb 4 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8568
ISSN (Print)1605-7422

Conference

ConferenceOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII
Country/TerritoryUnited States
CitySan Francisco, CA
Period02/2/1302/4/13

Keywords

  • fluorescence
  • nonmelanoma skin cancer
  • photodynamic therapy
  • PpIX

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