Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms

Tsung Li Liu; Srigokul Upadhyayula; Daniel E. Milkie; Ved Singh; Kai Wang; Ian A. Swinburne; Kishore R. Mosaliganti; Zach M. Collins; Tom W. Hiscock; Jamien Shea; Abraham Q. Kohrman; Taylor N. Medwig; Daphne Dambournet; Ryan Forster; Brian Cunniff; Yuan Ruan; Hanako Yashiro; Steffen Scholpp; Elliot M. Meyerowitz; Dirk Hockemeyer; David G. Drubin; Benjamin L. Martin; David Q. Matus; Minoru Koyama; Sean G. Megason; Tom Kirchhausen; Eric Betzig

doi:10.1126/science.aaq1392

Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms

Tsung Li Liu
, Srigokul Upadhyayula
, Daniel E. Milkie
, Ved Singh
, Kai Wang
, Ian A. Swinburne
, Kishore R. Mosaliganti
, Zach M. Collins
, Tom W. Hiscock
, Jamien Shea
, Abraham Q. Kohrman
, Taylor N. Medwig
, Daphne Dambournet
, Ryan Forster
, Brian Cunniff
, Yuan Ruan
, Hanako Yashiro
, Steffen Scholpp
, Elliot M. Meyerowitz
, Dirk Hockemeyer

David G. Drubin, Benjamin L. Martin, David Q. Matus, Minoru Koyama, Sean G. Megason, Tom Kirchhausen, Eric Betzig

Biochemistry and Cell Biology

Howard Hughes Medical Institute
Harvard University
Boston Children's Hospital
Chinese Academy of Sciences
Stony Brook University
University of California at Berkeley
University of Vermont
California Institute of Technology
University of Exeter
Karlsruhe Institute of Technology

Research output: Contribution to journal › Article › peer-review

479 Scopus citations

Abstract

True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes that we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution, without inducing undue stress on either. We combined lattice light-sheet microscopy with adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.

Original language	English
Article number	eaaq1392
Journal	Science
Volume	360
Issue number	6386
DOIs	https://doi.org/10.1126/science.aaq1392
State	Published - Apr 20 2018

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Liu, T. L., Upadhyayula, S., Milkie, D. E., Singh, V., Wang, K., Swinburne, I. A., Mosaliganti, K. R., Collins, Z. M., Hiscock, T. W., Shea, J., Kohrman, A. Q., Medwig, T. N., Dambournet, D., Forster, R., Cunniff, B., Ruan, Y., Yashiro, H., Scholpp, S., Meyerowitz, E. M., ... Betzig, E. (2018). Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms. Science, 360(6386), Article eaaq1392. https://doi.org/10.1126/science.aaq1392

@article{befb339c28214f9b93d3e14083b9c8ab,

title = "Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms",

abstract = "True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes that we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution, without inducing undue stress on either. We combined lattice light-sheet microscopy with adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.",

author = "Liu, \{Tsung Li\} and Srigokul Upadhyayula and Milkie, \{Daniel E.\} and Ved Singh and Kai Wang and Swinburne, \{Ian A.\} and Mosaliganti, \{Kishore R.\} and Collins, \{Zach M.\} and Hiscock, \{Tom W.\} and Jamien Shea and Kohrman, \{Abraham Q.\} and Medwig, \{Taylor N.\} and Daphne Dambournet and Ryan Forster and Brian Cunniff and Yuan Ruan and Hanako Yashiro and Steffen Scholpp and Meyerowitz, \{Elliot M.\} and Dirk Hockemeyer and Drubin, \{David G.\} and Martin, \{Benjamin L.\} and Matus, \{David Q.\} and Minoru Koyama and Megason, \{Sean G.\} and Tom Kirchhausen and Eric Betzig",

note = "Publisher Copyright: {\textcopyright} 2017 The Authors.",

year = "2018",

month = apr,

day = "20",

doi = "10.1126/science.aaq1392",

language = "English",

volume = "360",

journal = "Science",

issn = "0036-8075",

number = "6386",

}

Liu, TL, Upadhyayula, S, Milkie, DE, Singh, V, Wang, K, Swinburne, IA, Mosaliganti, KR, Collins, ZM, Hiscock, TW, Shea, J, Kohrman, AQ, Medwig, TN, Dambournet, D, Forster, R, Cunniff, B, Ruan, Y, Yashiro, H, Scholpp, S, Meyerowitz, EM, Hockemeyer, D, Drubin, DG, Martin, BL, Matus, DQ, Koyama, M, Megason, SG, Kirchhausen, T & Betzig, E 2018, 'Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms', Science, vol. 360, no. 6386, eaaq1392. https://doi.org/10.1126/science.aaq1392

TY - JOUR

T1 - Observing the cell in its native state

T2 - Imaging subcellular dynamics in multicellular organisms

AU - Liu, Tsung Li

AU - Upadhyayula, Srigokul

AU - Milkie, Daniel E.

AU - Singh, Ved

AU - Wang, Kai

AU - Swinburne, Ian A.

AU - Mosaliganti, Kishore R.

AU - Collins, Zach M.

AU - Hiscock, Tom W.

AU - Shea, Jamien

AU - Kohrman, Abraham Q.

AU - Medwig, Taylor N.

AU - Dambournet, Daphne

AU - Forster, Ryan

AU - Cunniff, Brian

AU - Ruan, Yuan

AU - Yashiro, Hanako

AU - Scholpp, Steffen

AU - Meyerowitz, Elliot M.

AU - Hockemeyer, Dirk

AU - Drubin, David G.

AU - Martin, Benjamin L.

AU - Matus, David Q.

AU - Koyama, Minoru

AU - Megason, Sean G.

AU - Kirchhausen, Tom

AU - Betzig, Eric

PY - 2018/4/20

Y1 - 2018/4/20

N2 - True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes that we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution, without inducing undue stress on either. We combined lattice light-sheet microscopy with adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.

AB - True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes that we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution, without inducing undue stress on either. We combined lattice light-sheet microscopy with adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.

UR - https://www.scopus.com/pages/publications/85045622293

U2 - 10.1126/science.aaq1392

DO - 10.1126/science.aaq1392

M3 - Article

C2 - 29674564

AN - SCOPUS:85045622293

SN - 0036-8075

VL - 360

JO - Science

JF - Science

IS - 6386

M1 - eaaq1392

ER -

Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms

Abstract

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