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ON-CHIP INTEGRATION OF BIOLOGICAL SAMPLE BUFFER SWAP WITH DOWNSTREAM DIELECTROPHORETIC SEPARATION

  • University of Virginia

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Microfluidic cell enrichment based on electrophysiology phenotypes by dielectrophoresis requires that cells be resuspended from their physiological media into a lower conductivity buffer. To ensure that sensitive biological cells are not subject to centrifugation and spend minimal time outside of their optimal culture media, we present on-chip integration of sample preparation to dielectrophoretic separation. A microfluidic device with tangential flows is presented for transferring cells into a media with 100-fold lower conductivity, while minimizing loss of cell concentration and the output sample flow rate is validated for downstream dielectrophoretic deflection.

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages45-46
Number of pages2
ISBN (Electronic)9781733419031
StatePublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: Oct 10 2021Oct 14 2021

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period10/10/2110/14/21

Keywords

  • Dielectrophoresis
  • Diffusional Mixing
  • Flow Dispersion
  • On-chip Integration
  • Sample Preparation

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