One β hairpin after the other: Exploring mechanical unfolding pathways of the transmembrane β-barrel protein ompg

K. Tanuj Sapra; Mehdi Damaghi; Stefan Köster; Özkan Yildiz; Werner Kühlbrandt; Daniel J. Müller

doi:10.1002/anie.200904361

One β hairpin after the other: Exploring mechanical unfolding pathways of the transmembrane β-barrel protein ompg

K. Tanuj Sapra
, Mehdi Damaghi
, Stefan Köster
, Özkan Yildiz
, Werner Kühlbrandt
, Daniel J. Müller

Pathology

Technische Universität Dresden
University of Oxford

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Roll out the barrel: By using singlemolecule force spectroscopy, a ß-barrelforming outer-membrane protein is unfolded for the first time. OmpC from E coli shows a surprising unfolding behavior: Singleβ, strands do not unfold individually but as β hairpins. These ß hairpins unfold one after another until the entire β-barrel membrane protein is unfolded (see structural representation).

Original language	English
Pages (from-to)	8306-8308
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	48
Issue number	44
DOIs	https://doi.org/10.1002/anie.200904361
State	Published - Oct 19 2009

Keywords

Barrel structures
Mechanical unfolding
Membrane proteins
Molecular interactions
Protein unfolding

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10.1002/anie.200904361

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abstract = "Roll out the barrel: By using singlemolecule force spectroscopy, a {\ss}-barrelforming outer-membrane protein is unfolded for the first time. OmpC from E coli shows a surprising unfolding behavior: Singleβ, strands do not unfold individually but as β hairpins. These {\ss} hairpins unfold one after another until the entire β-barrel membrane protein is unfolded (see structural representation).",

keywords = "Barrel structures, Mechanical unfolding, Membrane proteins, Molecular interactions, Protein unfolding",

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AU - Köster, Stefan

AU - Yildiz, Özkan

AU - Kühlbrandt, Werner

AU - Müller, Daniel J.

PY - 2009/10/19

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KW - Mechanical unfolding

KW - Membrane proteins

KW - Molecular interactions

KW - Protein unfolding

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One β hairpin after the other: Exploring mechanical unfolding pathways of the transmembrane β-barrel protein ompg

Abstract

Keywords

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