OpenSTED: Inexpensive and open-source implementation of dynamic intensity minimum (DyMIN) for stimulated emission depletion (STED) microscopy

Stephanie A. Meyer; Mark E. Siemens; Juliet T. Gopinath; Diego Restrepo; Emily A. Gibson

OpenSTED: Inexpensive and open-source implementation of dynamic intensity minimum (DyMIN) for stimulated emission depletion (STED) microscopy

Stephanie A. Meyer
, Mark E. Siemens
, Juliet T. Gopinath
, Diego Restrepo
, Emily A. Gibson

Physiology and Biophysics

University of Colorado Denver
University of Denver
University of Colorado Boulder

Research output: Contribution to journal › Conference article › peer-review

Abstract

The DyMIN method reduces photobleaching, a problem in STED microscopy. Labs implementing custom-built STED microscopes would greatly benefit from DyMIN capabilities. We present an inexpensive, open-source version utilizing an FPGA and multiplexer.

Original language	English
Article number	NW4C.3
Journal	Optics InfoBase Conference Papers
State	Published - 2021
Event	Novel Techniques in Microscopy, NTM 2021 - Part of Biophotonics Congress: Optics in the Life Sciences 2021 - Virtual, Online, United States Duration: Apr 12 2021 → Apr 16 2021

Cite this

@article{fd5424447c6149b3a2fe6143a7c9cfd9,

title = "OpenSTED: Inexpensive and open-source implementation of dynamic intensity minimum (DyMIN) for stimulated emission depletion (STED) microscopy",

abstract = "The DyMIN method reduces photobleaching, a problem in STED microscopy. Labs implementing custom-built STED microscopes would greatly benefit from DyMIN capabilities. We present an inexpensive, open-source version utilizing an FPGA and multiplexer.",

author = "Meyer, \{Stephanie A.\} and Siemens, \{Mark E.\} and Gopinath, \{Juliet T.\} and Diego Restrepo and Gibson, \{Emily A.\}",

note = "Publisher Copyright: {\textcopyright} 2021 The Author (s).; Novel Techniques in Microscopy, NTM 2021 - Part of Biophotonics Congress: Optics in the Life Sciences 2021 ; Conference date: 12-04-2021 Through 16-04-2021",

year = "2021",

language = "English",

journal = "Optics InfoBase Conference Papers",

issn = "2162-2701",

}

TY - JOUR

T1 - OpenSTED

T2 - Novel Techniques in Microscopy, NTM 2021 - Part of Biophotonics Congress: Optics in the Life Sciences 2021

AU - Meyer, Stephanie A.

AU - Siemens, Mark E.

AU - Gopinath, Juliet T.

AU - Restrepo, Diego

AU - Gibson, Emily A.

PY - 2021

Y1 - 2021

N2 - The DyMIN method reduces photobleaching, a problem in STED microscopy. Labs implementing custom-built STED microscopes would greatly benefit from DyMIN capabilities. We present an inexpensive, open-source version utilizing an FPGA and multiplexer.

AB - The DyMIN method reduces photobleaching, a problem in STED microscopy. Labs implementing custom-built STED microscopes would greatly benefit from DyMIN capabilities. We present an inexpensive, open-source version utilizing an FPGA and multiplexer.

UR - https://www.scopus.com/pages/publications/85119680899

M3 - Conference article

AN - SCOPUS:85119680899

SN - 2162-2701

JO - Optics InfoBase Conference Papers

JF - Optics InfoBase Conference Papers

M1 - NW4C.3

Y2 - 12 April 2021 through 16 April 2021

ER -

OpenSTED: Inexpensive and open-source implementation of dynamic intensity minimum (DyMIN) for stimulated emission depletion (STED) microscopy

Abstract

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