Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage Decisions in Developing Embryoid Bodies

Ik Soo Kim; Jingyi Wu; Gilbert J. Rahme; Sofia Battaglia; Atray Dixit; Elizabeth Gaskell; Huidong Chen; Luca Pinello; Bradley E. Bernstein

doi:10.1016/j.celrep.2020.108222

Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage Decisions in Developing Embryoid Bodies

Ik Soo Kim
, Jingyi Wu
, Gilbert J. Rahme
, Sofia Battaglia
, Atray Dixit
, Elizabeth Gaskell
, Huidong Chen
, Luca Pinello
, Bradley E. Bernstein

Massachusetts General Hospital
Broad Institute

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Kim et al. present a temporally precise genetic recording system for lineage tracing and transcriptomics analysis of single cells. They generate a trajectory map and single-cell transcriptional atlas of developing embryoid bodies, an organoid model of pre-gastrulation embryogenesis. These data reveal transcriptional and epigenetic regulators of early cell fate decisions.

Original language	English
Article number	108222
Journal	Cell Reports
Volume	33
Issue number	1
DOIs	https://doi.org/10.1016/j.celrep.2020.108222
State	Published - Oct 6 2020

Keywords

differentiation
embryogenesis
lineage tracing
single-cell RNA sequencing
stem cells

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10.1016/j.celrep.2020.108222

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abstract = "Kim et al. present a temporally precise genetic recording system for lineage tracing and transcriptomics analysis of single cells. They generate a trajectory map and single-cell transcriptional atlas of developing embryoid bodies, an organoid model of pre-gastrulation embryogenesis. These data reveal transcriptional and epigenetic regulators of early cell fate decisions.",

keywords = "differentiation, embryogenesis, lineage tracing, single-cell RNA sequencing, stem cells",

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AU - Kim, Ik Soo

AU - Wu, Jingyi

AU - Rahme, Gilbert J.

AU - Battaglia, Sofia

AU - Dixit, Atray

AU - Gaskell, Elizabeth

AU - Chen, Huidong

AU - Pinello, Luca

AU - Bernstein, Bradley E.

PY - 2020/10/6

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AB - Kim et al. present a temporally precise genetic recording system for lineage tracing and transcriptomics analysis of single cells. They generate a trajectory map and single-cell transcriptional atlas of developing embryoid bodies, an organoid model of pre-gastrulation embryogenesis. These data reveal transcriptional and epigenetic regulators of early cell fate decisions.

KW - differentiation

KW - embryogenesis

KW - lineage tracing

KW - single-cell RNA sequencing

KW - stem cells

UR - https://www.scopus.com/pages/publications/85091902244

U2 - 10.1016/j.celrep.2020.108222

DO - 10.1016/j.celrep.2020.108222

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Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage Decisions in Developing Embryoid Bodies

Abstract

Keywords

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