Partial purification of a rat liver enzyme that catalyzes the formation of fructose 2,6-bisphosphate

M. R. El-Maghrabi; T. H. Claus; J. Pilkis; S. J. Pilkis

doi:10.1016/0006-291X(81)91858-1

Partial purification of a rat liver enzyme that catalyzes the formation of fructose 2,6-bisphosphate

M. R. El-Maghrabi
, T. H. Claus
, J. Pilkis
, S. J. Pilkis

Physiology and Biophysics

Vanderbilt University

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

A 6-phosphofructo 2-kinase (ATP:D-fructose-6-phosphate 2-phosphotransferase) activity was detected in rat liver cytosol. The enzyme was partially purified by (NH₄)₂SO₄ fractionation, DEAE-Cellulose chromatography, and gel filtration on Sephadex G-100. This enzyme catalyzed the transfer of phosphate from ATP to the C2 position of fructose 6-phosphate. The apparent K_m for fructose 6-phosphate was 0.5 mM while that for ATP was 0.2 mM. It is suggested that fructose 2,6-bisphosphate is synthesized via this unique enzyme reaction.

Original language	English
Pages (from-to)	1071-1077
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	101
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(81)91858-1
State	Published - Aug 14 1981

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title = "Partial purification of a rat liver enzyme that catalyzes the formation of fructose 2,6-bisphosphate",

abstract = "A 6-phosphofructo 2-kinase (ATP:D-fructose-6-phosphate 2-phosphotransferase) activity was detected in rat liver cytosol. The enzyme was partially purified by (NH4)2SO4 fractionation, DEAE-Cellulose chromatography, and gel filtration on Sephadex G-100. This enzyme catalyzed the transfer of phosphate from ATP to the C2 position of fructose 6-phosphate. The apparent Km for fructose 6-phosphate was 0.5 mM while that for ATP was 0.2 mM. It is suggested that fructose 2,6-bisphosphate is synthesized via this unique enzyme reaction.",

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T1 - Partial purification of a rat liver enzyme that catalyzes the formation of fructose 2,6-bisphosphate

AU - El-Maghrabi, M. R.

AU - Claus, T. H.

AU - Pilkis, J.

AU - Pilkis, S. J.

PY - 1981/8/14

Y1 - 1981/8/14

N2 - A 6-phosphofructo 2-kinase (ATP:D-fructose-6-phosphate 2-phosphotransferase) activity was detected in rat liver cytosol. The enzyme was partially purified by (NH4)2SO4 fractionation, DEAE-Cellulose chromatography, and gel filtration on Sephadex G-100. This enzyme catalyzed the transfer of phosphate from ATP to the C2 position of fructose 6-phosphate. The apparent Km for fructose 6-phosphate was 0.5 mM while that for ATP was 0.2 mM. It is suggested that fructose 2,6-bisphosphate is synthesized via this unique enzyme reaction.

AB - A 6-phosphofructo 2-kinase (ATP:D-fructose-6-phosphate 2-phosphotransferase) activity was detected in rat liver cytosol. The enzyme was partially purified by (NH4)2SO4 fractionation, DEAE-Cellulose chromatography, and gel filtration on Sephadex G-100. This enzyme catalyzed the transfer of phosphate from ATP to the C2 position of fructose 6-phosphate. The apparent Km for fructose 6-phosphate was 0.5 mM while that for ATP was 0.2 mM. It is suggested that fructose 2,6-bisphosphate is synthesized via this unique enzyme reaction.

UR - https://www.scopus.com/pages/publications/0019879836

U2 - 10.1016/0006-291X(81)91858-1

DO - 10.1016/0006-291X(81)91858-1

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AN - SCOPUS:0019879836

SN - 0006-291X

VL - 101

SP - 1071

EP - 1077

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Partial purification of a rat liver enzyme that catalyzes the formation of fructose 2,6-bisphosphate

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