Peripheral blood mononuclear cell infiltration and neuroinflammation in the HexB-/- mouse model of neurodegeneration

Stephanos Kyrkanides; Ann W. Miller; Jen nie H. Miller; Ross H. Tallents; Sabine M. Brouxhon; Mallory E. Olschowka; M. Kerry O'Banion; John A. Olschowka

doi:10.1016/j.jneuroim.2008.06.024

Peripheral blood mononuclear cell infiltration and neuroinflammation in the HexB^-/- mouse model of neurodegeneration

Stephanos Kyrkanides
, Ann W. Miller
, Jen nie H. Miller
, Ross H. Tallents
, Sabine M. Brouxhon
, Mallory E. Olschowka
, M. Kerry O'Banion
, John A. Olschowka

Physiology and Biophysics

University of Rochester

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Myeloid-derived immune cells, including microglia, macrophages and monocytes, have been previously implicated in neurodegeneration. We investigated the role of infiltrating peripheral blood mononuclear cells (PBMC) in neuroinflammation and neurodegeneration in the HexB^-/- mouse model of Sandhoff disease. Ablation of the chemokine receptor CCR2 in the HexB^-/- mouse resulted in significant inhibition of PBMC infiltration into the brain, decrease in TNFα and MHC-II mRNA abundance and retardation in clinical disease development. There was no change in the level of GM₂ storage and pro-apoptotic activity or astrocyte activation in HexB^-/-;Ccr2^-/- double knockout mice, which eventually succumbed secondary to GM₂ gangliosidosis.

Original language	English
Pages (from-to)	50-57
Number of pages	8
Journal	Journal of Neuroimmunology
Volume	203
Issue number	1
DOIs	https://doi.org/10.1016/j.jneuroim.2008.06.024
State	Published - Oct 15 2008

Keywords

CCR2
Chemokine receptors
Microglia
Neurodegeneration
Neuroinflammation
Peripheral blood
Sandhoff disease

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10.1016/j.jneuroim.2008.06.024

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title = "Peripheral blood mononuclear cell infiltration and neuroinflammation in the HexB-/- mouse model of neurodegeneration",

abstract = "Myeloid-derived immune cells, including microglia, macrophages and monocytes, have been previously implicated in neurodegeneration. We investigated the role of infiltrating peripheral blood mononuclear cells (PBMC) in neuroinflammation and neurodegeneration in the HexB-/- mouse model of Sandhoff disease. Ablation of the chemokine receptor CCR2 in the HexB-/- mouse resulted in significant inhibition of PBMC infiltration into the brain, decrease in TNFα and MHC-II mRNA abundance and retardation in clinical disease development. There was no change in the level of GM2 storage and pro-apoptotic activity or astrocyte activation in HexB-/-;Ccr2-/- double knockout mice, which eventually succumbed secondary to GM2 gangliosidosis.",

keywords = "CCR2, Chemokine receptors, Microglia, Neurodegeneration, Neuroinflammation, Peripheral blood, Sandhoff disease",

author = "Stephanos Kyrkanides and Miller, \{Ann W.\} and Miller, \{Jen nie H.\} and Tallents, \{Ross H.\} and Brouxhon, \{Sabine M.\} and Olschowka, \{Mallory E.\} and O'Banion, \{M. Kerry\} and Olschowka, \{John A.\}",

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doi = "10.1016/j.jneuroim.2008.06.024",

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TY - JOUR

T1 - Peripheral blood mononuclear cell infiltration and neuroinflammation in the HexB-/- mouse model of neurodegeneration

AU - Kyrkanides, Stephanos

AU - Miller, Ann W.

AU - Miller, Jen nie H.

AU - Tallents, Ross H.

AU - Brouxhon, Sabine M.

AU - Olschowka, Mallory E.

AU - O'Banion, M. Kerry

AU - Olschowka, John A.

PY - 2008/10/15

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N2 - Myeloid-derived immune cells, including microglia, macrophages and monocytes, have been previously implicated in neurodegeneration. We investigated the role of infiltrating peripheral blood mononuclear cells (PBMC) in neuroinflammation and neurodegeneration in the HexB-/- mouse model of Sandhoff disease. Ablation of the chemokine receptor CCR2 in the HexB-/- mouse resulted in significant inhibition of PBMC infiltration into the brain, decrease in TNFα and MHC-II mRNA abundance and retardation in clinical disease development. There was no change in the level of GM2 storage and pro-apoptotic activity or astrocyte activation in HexB-/-;Ccr2-/- double knockout mice, which eventually succumbed secondary to GM2 gangliosidosis.

AB - Myeloid-derived immune cells, including microglia, macrophages and monocytes, have been previously implicated in neurodegeneration. We investigated the role of infiltrating peripheral blood mononuclear cells (PBMC) in neuroinflammation and neurodegeneration in the HexB-/- mouse model of Sandhoff disease. Ablation of the chemokine receptor CCR2 in the HexB-/- mouse resulted in significant inhibition of PBMC infiltration into the brain, decrease in TNFα and MHC-II mRNA abundance and retardation in clinical disease development. There was no change in the level of GM2 storage and pro-apoptotic activity or astrocyte activation in HexB-/-;Ccr2-/- double knockout mice, which eventually succumbed secondary to GM2 gangliosidosis.

KW - CCR2

KW - Chemokine receptors

KW - Microglia

KW - Neurodegeneration

KW - Neuroinflammation

KW - Peripheral blood

KW - Sandhoff disease

UR - https://www.scopus.com/pages/publications/52949130599

U2 - 10.1016/j.jneuroim.2008.06.024

DO - 10.1016/j.jneuroim.2008.06.024

M3 - Article

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AN - SCOPUS:52949130599

SN - 0165-5728

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JO - Journal of Neuroimmunology

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ER -

Peripheral blood mononuclear cell infiltration and neuroinflammation in the HexB^-/- mouse model of neurodegeneration

Abstract

Keywords

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