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Phase contrast image restoration via dictionary representation of diffraction patterns

  • Shanghai Jiao Tong University
  • Carnegie Mellon University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

25 Scopus citations

Abstract

The restoration of microscopy images makes the segmentation and detection of cells easier and more reliable, which facilitates automated cell tracking and cell behavior analysis. In this paper, the authors analyze the image formation process of phase contrast images and propose an image restoration method based on the dictionary representation of diffraction patterns. By formulating and solving a min-ℓ1 optimization problem, each pixel is restored into a feature vector corresponding to the dictionary representation. Cells in the images are then segmented by the feature vector clustering. In addition to segmentation, since the feature vectors capture the information on the phase retardation caused by cells, they can be used for cell stage classification between intermitotic and mitotic/apoptotic stages. Experiments on three image sequences demonstrate that the dictionary-based restoration method can restore phase contrast images containing cells with different optical natures and provide promising results on cell stage classification.

Original languageEnglish
Title of host publicationMedical Image Computing and Computer-Assisted Intervention, MICCAI2012 - 15th International Conference, Proceedings
EditorsNicholas Ayache, Herve Delingette, Polina Golland, Kensaku Mori
PublisherSpringer Verlag
Pages615-622
Number of pages8
ISBN (Print)9783642334535
DOIs
StatePublished - 2012
Event15th International Conference on Medical Image Computing and Computer Assisted Intervention, MICCAI 2012 - Nice, France
Duration: Oct 1 2012Oct 5 2012

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
Volume7512 LNCS
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349

Conference

Conference15th International Conference on Medical Image Computing and Computer Assisted Intervention, MICCAI 2012
Country/TerritoryFrance
CityNice
Period10/1/1210/5/12

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