Phorbol myristate acetate-dependent association of protein kinase C α with phospholipase D1 in intact cells

Taehoon G. Lee; Jong Bae Park; Sang Do Lee; Seungbum Hong; Jae Ho Kim; Yong Kim; Kye Sook Yi; Sunsik Bae; Yusuf A. Hannun; Lina M. Obeid; Pann Ghill Suh; Sung Ho Ryu

doi:10.1016/S0005-2760(97)00083-0

Phorbol myristate acetate-dependent association of protein kinase C α with phospholipase D1 in intact cells

Taehoon G. Lee
, Jong Bae Park
, Sang Do Lee
, Seungbum Hong
, Jae Ho Kim
, Yong Kim
, Kye Sook Yi
, Sunsik Bae
, Yusuf A. Hannun
, Lina M. Obeid
, Pann Ghill Suh
, Sung Ho Ryu

Cancer Center

Pohang University of Science and Technology
Duke University

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

A phospholipase D1 (PLD1) was purified from rat brain by the use of antibody-coupled protein A Sepharose. We found that protein kinase Cα (PKCα)) stimulated PLD1 activity in the presence of phorbol myristate acetate (PMA). PMA-dependent association of PKCα with PLD1 was verified in NIH-3T3 fibroblast cells, and COS7 cells transiently expressing PLD1 as well as in vitro suggesting that the activation of PLD1 resulted from direct association of PKCα with PLD1.

Original language	English
Pages (from-to)	199-204
Number of pages	6
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	1347
Issue number	2-3
DOIs	https://doi.org/10.1016/S0005-2760(97)00083-0
State	Published - Aug 16 1997

Keywords

Antibody
Immunoaffinity-purification
Immunoprecipitation
Phorbol myristate acetate
Phospholipase D1
Protein kinase Cα

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10.1016/S0005-2760(97)00083-0

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Lee, T. G., Park, J. B., Lee, S. D., Hong, S., Kim, J. H., Kim, Y., Yi, K. S., Bae, S., Hannun, Y. A., Obeid, L. M., Suh, P. G., & Ryu, S. H. (1997). Phorbol myristate acetate-dependent association of protein kinase C α with phospholipase D1 in intact cells. Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, 1347(2-3), 199-204. https://doi.org/10.1016/S0005-2760(97)00083-0

@article{69a5f4cb536e4af5a5f0e3d20a2f739d,

title = "Phorbol myristate acetate-dependent association of protein kinase C α with phospholipase D1 in intact cells",

abstract = "A phospholipase D1 (PLD1) was purified from rat brain by the use of antibody-coupled protein A Sepharose. We found that protein kinase Cα (PKCα)) stimulated PLD1 activity in the presence of phorbol myristate acetate (PMA). PMA-dependent association of PKCα with PLD1 was verified in NIH-3T3 fibroblast cells, and COS7 cells transiently expressing PLD1 as well as in vitro suggesting that the activation of PLD1 resulted from direct association of PKCα with PLD1.",

keywords = "Antibody, Immunoaffinity-purification, Immunoprecipitation, Phorbol myristate acetate, Phospholipase D1, Protein kinase Cα",

author = "Lee, \{Taehoon G.\} and Park, \{Jong Bae\} and Lee, \{Sang Do\} and Seungbum Hong and Kim, \{Jae Ho\} and Yong Kim and Yi, \{Kye Sook\} and Sunsik Bae and Hannun, \{Yusuf A.\} and Obeid, \{Lina M.\} and Suh, \{Pann Ghill\} and Ryu, \{Sung Ho\}",

year = "1997",

month = aug,

day = "16",

doi = "10.1016/S0005-2760(97)00083-0",

language = "English",

volume = "1347",

pages = "199--204",

journal = "Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism",

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Lee, TG, Park, JB, Lee, SD, Hong, S, Kim, JH, Kim, Y, Yi, KS, Bae, S, Hannun, YA, Obeid, LM, Suh, PG & Ryu, SH 1997, 'Phorbol myristate acetate-dependent association of protein kinase C α with phospholipase D1 in intact cells', Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, vol. 1347, no. 2-3, pp. 199-204. https://doi.org/10.1016/S0005-2760(97)00083-0

TY - JOUR

T1 - Phorbol myristate acetate-dependent association of protein kinase C α with phospholipase D1 in intact cells

AU - Lee, Taehoon G.

AU - Park, Jong Bae

AU - Lee, Sang Do

AU - Hong, Seungbum

AU - Kim, Jae Ho

AU - Kim, Yong

AU - Yi, Kye Sook

AU - Bae, Sunsik

AU - Hannun, Yusuf A.

AU - Obeid, Lina M.

AU - Suh, Pann Ghill

AU - Ryu, Sung Ho

PY - 1997/8/16

Y1 - 1997/8/16

N2 - A phospholipase D1 (PLD1) was purified from rat brain by the use of antibody-coupled protein A Sepharose. We found that protein kinase Cα (PKCα)) stimulated PLD1 activity in the presence of phorbol myristate acetate (PMA). PMA-dependent association of PKCα with PLD1 was verified in NIH-3T3 fibroblast cells, and COS7 cells transiently expressing PLD1 as well as in vitro suggesting that the activation of PLD1 resulted from direct association of PKCα with PLD1.

AB - A phospholipase D1 (PLD1) was purified from rat brain by the use of antibody-coupled protein A Sepharose. We found that protein kinase Cα (PKCα)) stimulated PLD1 activity in the presence of phorbol myristate acetate (PMA). PMA-dependent association of PKCα with PLD1 was verified in NIH-3T3 fibroblast cells, and COS7 cells transiently expressing PLD1 as well as in vitro suggesting that the activation of PLD1 resulted from direct association of PKCα with PLD1.

KW - Antibody

KW - Immunoaffinity-purification

KW - Immunoprecipitation

KW - Phorbol myristate acetate

KW - Phospholipase D1

KW - Protein kinase Cα

UR - https://www.scopus.com/pages/publications/0031575184

U2 - 10.1016/S0005-2760(97)00083-0

DO - 10.1016/S0005-2760(97)00083-0

M3 - Article

C2 - 9295164

AN - SCOPUS:0031575184

SN - 0005-2760

VL - 1347

SP - 199

EP - 204

JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism

JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism

IS - 2-3

ER -

Phorbol myristate acetate-dependent association of protein kinase C α with phospholipase D1 in intact cells

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