Phosphorus NMR analysis of phospholipids in detergents

E. London; G. W. Feigenson

Phosphorus NMR analysis of phospholipids in detergents

E. London
, G. W. Feigenson

Biochemistry and Cell Biology

Cornell University

Research output: Contribution to journal › Article › peer-review

124 Scopus citations

Abstract

Various detergents can be used to dissolve phospholipids, resulting in vary narrow ³¹PNMR resonances. These resonances are well resolved, allowing identification and quantitative analysis of phospholipids in a mixture. The chemical shift depends strongly on pH, reflecting changes in the state of ionization of the phospholipid headgroup moieties. Samples of phospholipids dissolved in aqueous detergents are conveniently prepared and give narrower ³¹P resonances than do phospholipids dissolved in organic solvents.

Original language	English
Pages (from-to)	408-412
Number of pages	5
Journal	Journal of Lipid Research
Volume	20
Issue number	3
State	Published - 1979

Cite this

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title = "Phosphorus NMR analysis of phospholipids in detergents",

abstract = "Various detergents can be used to dissolve phospholipids, resulting in vary narrow 31PNMR resonances. These resonances are well resolved, allowing identification and quantitative analysis of phospholipids in a mixture. The chemical shift depends strongly on pH, reflecting changes in the state of ionization of the phospholipid headgroup moieties. Samples of phospholipids dissolved in aqueous detergents are conveniently prepared and give narrower 31P resonances than do phospholipids dissolved in organic solvents.",

author = "E. London and Feigenson, \{G. W.\}",

year = "1979",

language = "English",

volume = "20",

pages = "408--412",

journal = "Journal of Lipid Research",

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TY - JOUR

T1 - Phosphorus NMR analysis of phospholipids in detergents

AU - London, E.

AU - Feigenson, G. W.

PY - 1979

Y1 - 1979

N2 - Various detergents can be used to dissolve phospholipids, resulting in vary narrow 31PNMR resonances. These resonances are well resolved, allowing identification and quantitative analysis of phospholipids in a mixture. The chemical shift depends strongly on pH, reflecting changes in the state of ionization of the phospholipid headgroup moieties. Samples of phospholipids dissolved in aqueous detergents are conveniently prepared and give narrower 31P resonances than do phospholipids dissolved in organic solvents.

AB - Various detergents can be used to dissolve phospholipids, resulting in vary narrow 31PNMR resonances. These resonances are well resolved, allowing identification and quantitative analysis of phospholipids in a mixture. The chemical shift depends strongly on pH, reflecting changes in the state of ionization of the phospholipid headgroup moieties. Samples of phospholipids dissolved in aqueous detergents are conveniently prepared and give narrower 31P resonances than do phospholipids dissolved in organic solvents.

UR - https://www.scopus.com/pages/publications/0018742678

M3 - Article

C2 - 36440

AN - SCOPUS:0018742678

SN - 0022-2275

VL - 20

SP - 408

EP - 412

JO - Journal of Lipid Research

JF - Journal of Lipid Research

IS - 3

ER -

Phosphorus NMR analysis of phospholipids in detergents

Abstract

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