Physical activity engendering loads from diverse directions augments the growing skeleton

METHODS: Growing female C57BL/6J mice were housed individually for 3 months in enclosures designed to accentuate either non-linear locomotion (diverse-orientation loading) or linear locomotion (stereotypic-orientation loading) (n=10/cage type). Behavioral assessments were performed daily to quantify cage activity level. Following the experiment, trabecular and cortical bone structure in the humeral head and distal femoral metaphysis were analyzed with μCT.

RESULTS: Throughout the experiment, groups did not differ in cage activity level. Yet, following the experiment, the proximal humeri of mice that experienced increased diverse-orientation loading had significantly greater trabecular bone volume fraction (p=0.004), greater cortical bone area (p=0.005), greater cortical area fraction (p=0.0007), and thicker cortices (p=0.002). No significant group differences were detected in the distal femoral metaphysis.

CONCLUSIONS: Diverting habitual activities to entail loading from more diverse orientations can augment the growing mouse skeleton. This study suggests that low-intensity activities that produce loads from diverse directions may represent a viable alternative to vigorous, high-impact exercise as a means of benefiting skeletal health during growth.

OBJECTIVE: An experiment was conducted to determine if modifying habitual activities to involve mechanical loading from more diverse directions can enhance the growing skeleton.