Plasma Microbial Cell-free DNA Sequencing for Diagnosis of Pediatric Lyme Disease

Andrew S. Handel; Asim A. Ahmed; Shivkumar Venkatasubrahmanyam; Sivan Bercovici; Qing Mao; Carine Ho; Desiree D. Hollemon; Christy Beneri

doi:10.1097/INF.0000000000004707

Plasma Microbial Cell-free DNA Sequencing for Diagnosis of Pediatric Lyme Disease

Andrew S. Handel
, Asim A. Ahmed
, Shivkumar Venkatasubrahmanyam
, Sivan Bercovici
, Qing Mao
, Carine Ho
, Desiree D. Hollemon
, Christy Beneri

Pediatrics

Karius, Inc.

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We conducted an exploratory study of plasma microbial cell-free DNA sequencing for the diagnosis of Lyme disease among pediatric patients. Low levels of Borrelia burgdorferi microbial cell-free DNA (<3–5 molecules per microliter) were observed in 6/9 serologically confirmed participants, including 4/5 with arthritis and 2/3 with multiple erythema migrans.

Original language	English
Pages (from-to)	473-475
Number of pages	3
Journal	Pediatric Infectious Disease Journal
Volume	44
Issue number	5
DOIs	https://doi.org/10.1097/INF.0000000000004707
State	Published - May 1 2025

Keywords

Borrelia burgdorferi
Lyme disease
next-generation sequencing

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10.1097/INF.0000000000004707

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abstract = "We conducted an exploratory study of plasma microbial cell-free DNA sequencing for the diagnosis of Lyme disease among pediatric patients. Low levels of Borrelia burgdorferi microbial cell-free DNA (<3–5 molecules per microliter) were observed in 6/9 serologically confirmed participants, including 4/5 with arthritis and 2/3 with multiple erythema migrans.",

keywords = "Borrelia burgdorferi, Lyme disease, next-generation sequencing",

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AU - Handel, Andrew S.

AU - Ahmed, Asim A.

AU - Venkatasubrahmanyam, Shivkumar

AU - Bercovici, Sivan

AU - Mao, Qing

AU - Ho, Carine

AU - Hollemon, Desiree D.

AU - Beneri, Christy

PY - 2025/5/1

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AB - We conducted an exploratory study of plasma microbial cell-free DNA sequencing for the diagnosis of Lyme disease among pediatric patients. Low levels of Borrelia burgdorferi microbial cell-free DNA (<3–5 molecules per microliter) were observed in 6/9 serologically confirmed participants, including 4/5 with arthritis and 2/3 with multiple erythema migrans.

KW - Borrelia burgdorferi

KW - Lyme disease

KW - next-generation sequencing

UR - https://www.scopus.com/pages/publications/85214991126

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Plasma Microbial Cell-free DNA Sequencing for Diagnosis of Pediatric Lyme Disease

Abstract

Keywords

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