pMPY-ZAP: A reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae

B. L. Schneider; B. Steiner; W. Seufert; A. B. Futcher

doi:10.1002/(SICI)1097-0061(199602)12:2<129::AID-YEA891>3.0.CO;2-O

pMPY-ZAP: A reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae

B. L. Schneider
, B. Steiner
, W. Seufert
, A. B. Futcher

Microbiology and Immunology

Cold Spring Harbor Laboratory
Ludwig Maximilian University of Munich

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.

Original language	English
Pages (from-to)	129-134
Number of pages	6
Journal	Yeast
Volume	12
Issue number	2
DOIs	https://doi.org/10.1002/(SICI)1097-0061(199602)12:2<129::AID-YEA891>3.0.CO;2-O
State	Published - Feb 1996

Keywords

Gene disruption
PCR-mediated disruption
Saccharomyces cerevisiae
Uraduction

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10.1002/(SICI)1097-0061(199602)12:2<129::AID-YEA891>3.0.CO;2-O

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abstract = "Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.",

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AU - Seufert, W.

AU - Futcher, A. B.

PY - 1996/2

Y1 - 1996/2

N2 - Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.

AB - Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.

KW - Gene disruption

KW - PCR-mediated disruption

KW - Saccharomyces cerevisiae

KW - Uraduction

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ER -

pMPY-ZAP: A reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae

Abstract

Keywords

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