Abstract
A temperature-sensitive polymer containing a terminal carboxyl group was prepared by copolymerization of N-isopropylacrylamide with mercaptoacetic acid. This polymer was conjugated to the IgG with EDCI and used to develop a novel polymer-mimetic enzyme competitive immunoassay method for determination of anti-IgG with iron-tetrasulfonatophthalocyanine as a labelling reagent to catalyze the reaction of p-hydroxyphenyl acetic acid (HPA) and hydrogen peroxide in alkaline medium. The calibration graph for anti-IgG was linear over the range of 0-1 500 ng/mL with a detection limit of 2. 0 ng/mL.
| Original language | English |
|---|---|
| Pages (from-to) | 758 |
| Number of pages | 1 |
| Journal | Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities |
| Volume | 22 |
| Issue number | 5 |
| State | Published - May 2001 |
Keywords
- Fluoroimmunoassay
- Heat-sensitive phase separation
- Mimetic enzyme
- Oligo-N-isopropylacrylamide
- Temperature-sensitive polymer
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