Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria)

Qianqian Liu; Bassem Allam; Jackie L. Collier

doi:10.1128/AEM.00246-09

Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria)

Stony Brook University

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.

Original language	English
Pages (from-to)	4913-4918
Number of pages	6
Journal	Applied and Environmental Microbiology
Volume	75
Issue number	14
DOIs	https://doi.org/10.1128/AEM.00246-09
State	Published - Jul 2009

Access to Document

10.1128/AEM.00246-09

Cite this

@article{8f25614d69ec456e8bff4f5ca7457e03,

title = "Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria)",

abstract = "We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.",

author = "Qianqian Liu and Bassem Allam and Collier, \{Jackie L.\}",

year = "2009",

month = jul,

doi = "10.1128/AEM.00246-09",

language = "English",

volume = "75",

pages = "4913--4918",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

number = "14",

}

TY - JOUR

T1 - Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria)

AU - Liu, Qianqian

AU - Allam, Bassem

AU - Collier, Jackie L.

PY - 2009/7

Y1 - 2009/7

N2 - We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.

AB - We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.

UR - https://www.scopus.com/pages/publications/67650500622

U2 - 10.1128/AEM.00246-09

DO - 10.1128/AEM.00246-09

M3 - Article

C2 - 19465523

AN - SCOPUS:67650500622

SN - 0099-2240

VL - 75

SP - 4913

EP - 4918

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 14

ER -

Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria)

Abstract

Access to Document

Other files and links

Fingerprint

Cite this