Skip to main navigation Skip to search Skip to main content

Rapid Amplification of cDNA Ends (RACE)

  • Stony Brook University

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

62 Scopus citations

Abstract

Rapid Amplification of cDNA ends (RACE) provides an inexpensive and powerful tool to quickly obtain full-length cDNA when the sequence is only partially known. Starting with an mRNA mixture, gene-specific primers generated from the known regions of the gene and non-specific anchors, full-length sequences can be identified in as little as 3 days. RACE can also be used to identify alternative transcripts of a gene when the partial or complete sequence of only one transcript is known. In the following sections, we outline details for rapid amplification of 5 and 3 cDNA ends using the “new RACE” technique.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-122
Number of pages16
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume703
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • alternative transcripts
  • new RACE
  • RACE

Fingerprint

Dive into the research topics of 'Rapid Amplification of cDNA Ends (RACE)'. Together they form a unique fingerprint.

Cite this