Real-Time Measurements of Cell Proliferation Using a Lab-on-CMOS Capacitance Sensor Array

Bathiya Prashan Senevirathna; Sheung Lu; Marc P. Dandin; John Basile; Elisabeth Smela; Pamela A. Abshire

doi:10.1109/TBCAS.2018.2821060

Real-Time Measurements of Cell Proliferation Using a Lab-on-CMOS Capacitance Sensor Array

Bathiya Prashan Senevirathna
, Sheung Lu
, Marc P. Dandin
, John Basile
, Elisabeth Smela
, Pamela A. Abshire

Electrical Engineering

University of Maryland, College Park
Kiskeya Microsystems LLC
University of Maryland, Baltimore

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

We describe a capacitance sensor array that has been incorporated into a lab-on-CMOS system for applications in monitoring cell viability. This paper presents analytical models, calibration results, and measured experimental results of the biosensor. The sensor has been characterized and exhibits a sensitivity of 590 kHz/fF. We report results from benchtop tests and in vitro experiments demonstrating on-chip tracking of cell adhesion as well as monitoring of cell viability. Human ovarian cancer cells were cultured on chip, and measured capacitance responses were validated by comparison with images from photomicrographs of the chip surface. Analysis was performed to quantify cell proliferation and adhesion, and responses to live cells were estimated to be 100 aF/cell.

Original language	English
Pages (from-to)	510-520
Number of pages	11
Journal	IEEE Transactions on Biomedical Circuits and Systems
Volume	12
Issue number	3
DOIs	https://doi.org/10.1109/TBCAS.2018.2821060
State	Published - Jun 2018

Keywords

Capacitance measurement
cell culture
CMOS integrated circuits
dielectric measurements
lab on a chip
mixed analog-digital integrated circuits

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10.1109/TBCAS.2018.2821060

Cite this

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title = "Real-Time Measurements of Cell Proliferation Using a Lab-on-CMOS Capacitance Sensor Array",

abstract = "We describe a capacitance sensor array that has been incorporated into a lab-on-CMOS system for applications in monitoring cell viability. This paper presents analytical models, calibration results, and measured experimental results of the biosensor. The sensor has been characterized and exhibits a sensitivity of 590 kHz/fF. We report results from benchtop tests and in vitro experiments demonstrating on-chip tracking of cell adhesion as well as monitoring of cell viability. Human ovarian cancer cells were cultured on chip, and measured capacitance responses were validated by comparison with images from photomicrographs of the chip surface. Analysis was performed to quantify cell proliferation and adhesion, and responses to live cells were estimated to be 100 aF/cell.",

keywords = "Capacitance measurement, cell culture, CMOS integrated circuits, dielectric measurements, lab on a chip, mixed analog-digital integrated circuits",

author = "Senevirathna, \{Bathiya Prashan\} and Sheung Lu and Dandin, \{Marc P.\} and John Basile and Elisabeth Smela and Abshire, \{Pamela A.\}",

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AU - Senevirathna, Bathiya Prashan

AU - Lu, Sheung

AU - Dandin, Marc P.

AU - Basile, John

AU - Smela, Elisabeth

AU - Abshire, Pamela A.

PY - 2018/6

Y1 - 2018/6

N2 - We describe a capacitance sensor array that has been incorporated into a lab-on-CMOS system for applications in monitoring cell viability. This paper presents analytical models, calibration results, and measured experimental results of the biosensor. The sensor has been characterized and exhibits a sensitivity of 590 kHz/fF. We report results from benchtop tests and in vitro experiments demonstrating on-chip tracking of cell adhesion as well as monitoring of cell viability. Human ovarian cancer cells were cultured on chip, and measured capacitance responses were validated by comparison with images from photomicrographs of the chip surface. Analysis was performed to quantify cell proliferation and adhesion, and responses to live cells were estimated to be 100 aF/cell.

AB - We describe a capacitance sensor array that has been incorporated into a lab-on-CMOS system for applications in monitoring cell viability. This paper presents analytical models, calibration results, and measured experimental results of the biosensor. The sensor has been characterized and exhibits a sensitivity of 590 kHz/fF. We report results from benchtop tests and in vitro experiments demonstrating on-chip tracking of cell adhesion as well as monitoring of cell viability. Human ovarian cancer cells were cultured on chip, and measured capacitance responses were validated by comparison with images from photomicrographs of the chip surface. Analysis was performed to quantify cell proliferation and adhesion, and responses to live cells were estimated to be 100 aF/cell.

KW - Capacitance measurement

KW - cell culture

KW - CMOS integrated circuits

KW - dielectric measurements

KW - lab on a chip

KW - mixed analog-digital integrated circuits

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JO - IEEE Transactions on Biomedical Circuits and Systems

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ER -

Real-Time Measurements of Cell Proliferation Using a Lab-on-CMOS Capacitance Sensor Array

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Keywords

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