Recognition and conversion of single- and double-stranded oligonucleotide substrates by 8-oxoguanine-DNA glycosylase from Escherichia coli

A. A. Ishchenko; N. V. Bulychev; G. A. Maksakova; F. Johnson; G. A. Nevinsky

Recognition and conversion of single- and double-stranded oligonucleotide substrates by 8-oxoguanine-DNA glycosylase from Escherichia coli

A. A. Ishchenko
, N. V. Bulychev
, G. A. Maksakova
, F. Johnson
, G. A. Nevinsky

Pharmacological Sciences

Siberian Div. of Russ. Acad. of Sci.

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The substrate specificity of 8-oxoguanine-DNA glycosylase was studied. The data showed for the first time that the enzyme can cleave single-stranded deoxyoligonucleotides containing an 8-oxoG link. The values of K_m and V_max for a range of single-stranded and double-stranded oligonucleotides (23 bp) containing 8-oxoG at different positions of one chain. These parameters consistently depended on the position of 8-oxoG in single-stranded or double-stranded substrates. A possible mechanism responsible for substrate recognition by 8-oxoguanine-DNA glycosylase is described.

Original language	English
Pages (from-to)	204-211
Number of pages	8
Journal	Biochemistry (Moscow)
Volume	62
Issue number	2
State	Published - 1997

Keywords

8-oxoguanine-DNA glycosylase
Single-stranded oligonucleotides
Substrate specificity

Cite this

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title = "Recognition and conversion of single- and double-stranded oligonucleotide substrates by 8-oxoguanine-DNA glycosylase from Escherichia coli",

abstract = "The substrate specificity of 8-oxoguanine-DNA glycosylase was studied. The data showed for the first time that the enzyme can cleave single-stranded deoxyoligonucleotides containing an 8-oxoG link. The values of Km and Vmax for a range of single-stranded and double-stranded oligonucleotides (23 bp) containing 8-oxoG at different positions of one chain. These parameters consistently depended on the position of 8-oxoG in single-stranded or double-stranded substrates. A possible mechanism responsible for substrate recognition by 8-oxoguanine-DNA glycosylase is described.",

keywords = "8-oxoguanine-DNA glycosylase, Single-stranded oligonucleotides, Substrate specificity",

author = "Ishchenko, \{A. A.\} and Bulychev, \{N. V.\} and Maksakova, \{G. A.\} and F. Johnson and Nevinsky, \{G. A.\}",

year = "1997",

language = "English",

volume = "62",

pages = "204--211",

journal = "Biochemistry (Moscow)",

issn = "0006-2979",

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TY - JOUR

T1 - Recognition and conversion of single- and double-stranded oligonucleotide substrates by 8-oxoguanine-DNA glycosylase from Escherichia coli

AU - Ishchenko, A. A.

AU - Bulychev, N. V.

AU - Maksakova, G. A.

AU - Johnson, F.

AU - Nevinsky, G. A.

PY - 1997

Y1 - 1997

N2 - The substrate specificity of 8-oxoguanine-DNA glycosylase was studied. The data showed for the first time that the enzyme can cleave single-stranded deoxyoligonucleotides containing an 8-oxoG link. The values of Km and Vmax for a range of single-stranded and double-stranded oligonucleotides (23 bp) containing 8-oxoG at different positions of one chain. These parameters consistently depended on the position of 8-oxoG in single-stranded or double-stranded substrates. A possible mechanism responsible for substrate recognition by 8-oxoguanine-DNA glycosylase is described.

AB - The substrate specificity of 8-oxoguanine-DNA glycosylase was studied. The data showed for the first time that the enzyme can cleave single-stranded deoxyoligonucleotides containing an 8-oxoG link. The values of Km and Vmax for a range of single-stranded and double-stranded oligonucleotides (23 bp) containing 8-oxoG at different positions of one chain. These parameters consistently depended on the position of 8-oxoG in single-stranded or double-stranded substrates. A possible mechanism responsible for substrate recognition by 8-oxoguanine-DNA glycosylase is described.

KW - 8-oxoguanine-DNA glycosylase

KW - Single-stranded oligonucleotides

KW - Substrate specificity

UR - https://www.scopus.com/pages/publications/0031063890

M3 - Article

C2 - 9159874

AN - SCOPUS:0031063890

SN - 0006-2979

VL - 62

SP - 204

EP - 211

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

IS - 2

ER -

Recognition and conversion of single- and double-stranded oligonucleotide substrates by 8-oxoguanine-DNA glycosylase from Escherichia coli

Abstract

Keywords

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