Resolving molecular orientation by polarization-sensitive stimulated Raman scattering microscopy

Pu Ting Dong; Cheng Zong; Ji Xin Cheng

doi:10.1016/B978-0-323-85158-9.00025-7

Resolving molecular orientation by polarization-sensitive stimulated Raman scattering microscopy

Pu Ting Dong
, Cheng Zong
, Ji Xin Cheng

Dept of Biomedical Engineering

Boston University

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

Polarization-sensitive stimulated Raman scattering (SRS) microscopy allows noninvasive, fast, and background-free vibrational imaging of highly ordered and oriented molecules. In this chapter, we describe the principle of polarization-sensitive SRS microscopy, the setup, recent applications, and its use for resolving amphotericin B orientation in fungal cell membrane. Polarization-sensitive SRS microscopy offers an advanced approach to study molecule orientation and assembly at submicron spatial resolution, which opens new opportunities for disease diagnosis and drug development.

Original language	English
Title of host publication	Stimulated Raman Scattering Microscopy
Subtitle of host publication	Techniques and Applications
Publisher	Elsevier
Pages	529-537
Number of pages	9
ISBN (Electronic)	9780323851589
ISBN (Print)	9780323903370
DOIs	https://doi.org/10.1016/B978-0-323-85158-9.00025-7
State	Published - Jan 1 2021

Keywords

Amphotericin B
Depolarization ratio
Orientation
Polarization
Stimulated raman scattering

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10.1016/B978-0-323-85158-9.00025-7

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abstract = "Polarization-sensitive stimulated Raman scattering (SRS) microscopy allows noninvasive, fast, and background-free vibrational imaging of highly ordered and oriented molecules. In this chapter, we describe the principle of polarization-sensitive SRS microscopy, the setup, recent applications, and its use for resolving amphotericin B orientation in fungal cell membrane. Polarization-sensitive SRS microscopy offers an advanced approach to study molecule orientation and assembly at submicron spatial resolution, which opens new opportunities for disease diagnosis and drug development.",

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AB - Polarization-sensitive stimulated Raman scattering (SRS) microscopy allows noninvasive, fast, and background-free vibrational imaging of highly ordered and oriented molecules. In this chapter, we describe the principle of polarization-sensitive SRS microscopy, the setup, recent applications, and its use for resolving amphotericin B orientation in fungal cell membrane. Polarization-sensitive SRS microscopy offers an advanced approach to study molecule orientation and assembly at submicron spatial resolution, which opens new opportunities for disease diagnosis and drug development.

KW - Amphotericin B

KW - Depolarization ratio

KW - Orientation

KW - Polarization

KW - Stimulated raman scattering

UR - https://www.scopus.com/pages/publications/85130667994

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BT - Stimulated Raman Scattering Microscopy

PB - Elsevier

ER -

Resolving molecular orientation by polarization-sensitive stimulated Raman scattering microscopy

Abstract

Keywords

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