TY - GEN
T1 - Retooling droplet microfluidics for the genomic analysis of a low number of single-cells
AU - Brouzes, E.
AU - Carniol, A.
AU - Bakowski, T.
AU - Strey, H.
N1 - Publisher Copyright:
© 14CBMS.
PY - 2014
Y1 - 2014
N2 - Our goal is to develop a droplet-based platform that performs the concurrent sample preparation of 100's of single-cells for genomic applications. Single-cell genomics remain low-throughput applications compared to the speed of droplet manipulations developed so far. We have devised a strategy to trade the high-throughput capacity of droplet technology for higher control of droplet manipulation. This strategy combines droplet, traps and on-chip valves. We have implemented a modular approach and we present here two novel modules that enable true single-cell encapsulation and interfacing droplet microfluidics with microplate technology respectively.
AB - Our goal is to develop a droplet-based platform that performs the concurrent sample preparation of 100's of single-cells for genomic applications. Single-cell genomics remain low-throughput applications compared to the speed of droplet manipulations developed so far. We have devised a strategy to trade the high-throughput capacity of droplet technology for higher control of droplet manipulation. This strategy combines droplet, traps and on-chip valves. We have implemented a modular approach and we present here two novel modules that enable true single-cell encapsulation and interfacing droplet microfluidics with microplate technology respectively.
KW - Droplet microfluidics
KW - Micro-to macro interface
KW - Single-cell encapsulation
KW - Single-cell genomics
UR - https://www.scopus.com/pages/publications/84941730202
M3 - Conference contribution
AN - SCOPUS:84941730202
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 1647
EP - 1649
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PB - Chemical and Biological Microsystems Society
T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Y2 - 26 October 2014 through 30 October 2014
ER -