Scaffolds with encapsulated DNA for non-viral gene delivery

A novel approach to create a biodegradable scaffold containing a condensed DNA core and a biodegradable polymer shell has been demonstrated. In this approach, the extended DNA chains were first collapsed into compact globules in a poor solvent, containing 94% DMF + 6%TE buffer, where the shrinkage in the radius of gyration was by a factor of about 9. The condensed DNA globule was then encapsulated by using a tri-block copolymer of polylactide-poly(ethylene glycol)-polylactide (LEL). The encapsulated DNA protected it from degradation during the electro-spinning process, which also removed the undesirable DMF solvent, preserving the DNA in the dry nanofibrous scaffold. An enhanced transfection activity was observed when the scaffold was used to deliver the embedded DNA to cells adhered to the scaffold.