Simultaneous X-ray fluorescence and ptychographic microscopy of Cyclotella meneghiniana

D. J. Vine; D. Pelliccia; C. Holzner; S. B. Baines; A. Berry; I. McNulty; S. Vogt; A. G. Peele; K. A. Nugent

doi:10.1364/OE.20.018287

Simultaneous X-ray fluorescence and ptychographic microscopy of Cyclotella meneghiniana

D. J. Vine
, D. Pelliccia
, C. Holzner
, S. B. Baines
, A. Berry
, I. McNulty
, S. Vogt
, A. G. Peele
, K. A. Nugent

Ecology and Evolution

Australian Research Council Centre of Excellence
University of Melbourne
United States Department of Energy
Monash University
XRADIA Inc.
La Trobe University
Australian Nuclear Science and Technology Organisation

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

Scanning X-ray fluorescence microscopy (XFM) is a particularly useful method for studying the spatial distribution of trace metals in biological samples. Here we demonstrate the utility of combining coherent diffractive imaging (CDI) with XFM for imaging biological samples to simultaneously produce high-resolution and high-contrast transmission images and quantitative elemental maps. The reconstructed transmission function yields morphological details which contextualise the elemental maps. We report enhancement of the spatial resolution in both the transmission and fluorescence images beyond that of the X-ray optics. The freshwater diatom Cyclotella meneghiniana was imaged to demonstrate the benefits of combining these techniques that have complementary contrast mechanisms.

Original language	English
Pages (from-to)	18287-18296
Number of pages	10
Journal	Optics Express
Volume	20
Issue number	16
DOIs	https://doi.org/10.1364/OE.20.018287
State	Published - Jul 30 2012

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10.1364/OE.20.018287

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abstract = "Scanning X-ray fluorescence microscopy (XFM) is a particularly useful method for studying the spatial distribution of trace metals in biological samples. Here we demonstrate the utility of combining coherent diffractive imaging (CDI) with XFM for imaging biological samples to simultaneously produce high-resolution and high-contrast transmission images and quantitative elemental maps. The reconstructed transmission function yields morphological details which contextualise the elemental maps. We report enhancement of the spatial resolution in both the transmission and fluorescence images beyond that of the X-ray optics. The freshwater diatom Cyclotella meneghiniana was imaged to demonstrate the benefits of combining these techniques that have complementary contrast mechanisms.",

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AU - Vine, D. J.

AU - Pelliccia, D.

AU - Holzner, C.

AU - Baines, S. B.

AU - Berry, A.

AU - McNulty, I.

AU - Vogt, S.

AU - Peele, A. G.

AU - Nugent, K. A.

PY - 2012/7/30

Y1 - 2012/7/30

N2 - Scanning X-ray fluorescence microscopy (XFM) is a particularly useful method for studying the spatial distribution of trace metals in biological samples. Here we demonstrate the utility of combining coherent diffractive imaging (CDI) with XFM for imaging biological samples to simultaneously produce high-resolution and high-contrast transmission images and quantitative elemental maps. The reconstructed transmission function yields morphological details which contextualise the elemental maps. We report enhancement of the spatial resolution in both the transmission and fluorescence images beyond that of the X-ray optics. The freshwater diatom Cyclotella meneghiniana was imaged to demonstrate the benefits of combining these techniques that have complementary contrast mechanisms.

AB - Scanning X-ray fluorescence microscopy (XFM) is a particularly useful method for studying the spatial distribution of trace metals in biological samples. Here we demonstrate the utility of combining coherent diffractive imaging (CDI) with XFM for imaging biological samples to simultaneously produce high-resolution and high-contrast transmission images and quantitative elemental maps. The reconstructed transmission function yields morphological details which contextualise the elemental maps. We report enhancement of the spatial resolution in both the transmission and fluorescence images beyond that of the X-ray optics. The freshwater diatom Cyclotella meneghiniana was imaged to demonstrate the benefits of combining these techniques that have complementary contrast mechanisms.

UR - https://www.scopus.com/pages/publications/84864600734

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DO - 10.1364/OE.20.018287

M3 - Article

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SN - 1094-4087

VL - 20

SP - 18287

EP - 18296

JO - Optics Express

JF - Optics Express

IS - 16

ER -

Simultaneous X-ray fluorescence and ptychographic microscopy of Cyclotella meneghiniana

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