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Single-cell detection of cytokines using a simple nanowell chip integrated with encoded microarray

  • Stony Brook University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Multiparameter analysis of individual immune cell's cytokine secretion has always been a challenging and complicated process that needs special facilities in a laboratory setting. Herein, we present an ultra-simple method with high sensitivity and high robustness to quantify cytokine expression at the single-cell resolution. A microchip with nanowell is integrated with a DNA-antibody convertible microarray. The sensitivity of the assay was found comparable to conventional methods. It has simplified a typically sophisticated multiplex single-cell assay into an instrument-free, point-of-detection technology, and thus it may find a broad utility in clinical diagnostics.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages474-475
Number of pages2
ISBN (Electronic)9781733419000
StatePublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: Oct 27 2019Oct 31 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/TerritorySwitzerland
CityBasel
Period10/27/1910/31/19

Keywords

  • Microchip
  • Multiplex
  • Point-of-care diagnosis
  • Simple method
  • Single-cell analysis

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