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Sphingosine-1-Phosphate Receptor 3 Potentiates Inflammatory Programs in Normal and Leukemia Stem Cells to Promote Differentiation

  • Stephanie Z. Xie
  • , Kerstin B. Kaufmann
  • , Weijia Wang
  • , Michelle Chan-Seng-Yue
  • , Olga I. Gan
  • , Elisa Laurenti
  • , Laura Garcia-Prat
  • , Shin Ichiro Takayanagi
  • , Stanley W.K. Ng
  • , Chang Jiang Xu
  • , Andy G.X. Zeng
  • , Liqing Jin
  • , Jessica McLeod
  • , Elvin Wagenblast
  • , Amanda Mitchell
  • , James A. Kennedy
  • , Qiang Liu
  • , Héléna Boutzen
  • , Melissa Kleinau
  • , Joseph Jargstorf
  • Gareth Holmes, Yang Zhang, Veronique Voisin, Gary D. Bader, Jean C.Y. Wang, Yusuf A. Hannun, Chiara Luberto, Timm Schroeder, Mark D. Minden, John E. Dick
  • Princess Margaret Cancer Centre
  • Swiss Federal Institute of Technology Zurich
  • Ontario Institute for Cancer Research
  • University of Cambridge
  • Kirin Holdings Co., Ltd.
  • University of Toronto
  • Centre of Excellence on Aging and Chronic Diseases of McGill Integrated University Health Network

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Acute myeloid leukemia (AML) is a caricature of normal hematopoiesis driven from leukemia stem cells (LSC) that share some hematopoietic stem cell (HSC) programs including responsiveness to inflammatory signaling. Although inflammation dysregulates mature myeloid cells and influences stemness programs and lineage determination in HSCs by activating stress myelopoiesis, such roles in LSCs are poorly understood. Here, we show that S1PR3, a receptor for the bioactive lipid sphingosine-1-phosphate, is a central regulator that drives myeloid differentiation and activates inflammatory programs in both HSCs and LSCs. S1PR3-mediated inflammatory signatures varied in a continuum from primitive to mature myeloid states across cohorts of patients with AML, each with distinct phenotypic and clinical properties. S1PR3 was high in LSCs and blasts of mature myeloid samples with linkages to chemosensitivity, whereas S1PR3 activation in primitive samples promoted LSC differentiation leading to eradication. Our studies open new avenues for therapeutic target identification specific for each AML subset.

Original languageEnglish
Pages (from-to)32-53
Number of pages22
JournalBlood Cancer Discovery
Volume2
Issue number1
DOIs
StatePublished - Jan 1 2021

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