Studies on Tetra-substituted Amino Aluminum Phthalocyanine as a New Red-region Substrate for Mimetic Peroxidase

Tetra-substituted amino aluminum phthalocyanine (TAAlPc) has been synthesized and used for the first time as a new red-region fluorescent substrate for the determination of hydrogen peroxide catalyzed by peroxidase or mimetic peroxidase. Under optimum conditions, the calibration graph has a linear range of 0. 0-3. 0 × 10^-7 mol/L H₂O₂ with a detection limit of 3. 7 × 10^-9 mol/L. The feasibility of TAAlPc as a new promising red-region substrate in practical application has been proven in the determination of H₂O₂ in rainwater. The proposed method can largely minimize the interference that results from background fluorescence or scattering light and has a high analytical sensitivity.