@inproceedings{edfcfe4869154c25b61b9fd39deb7894,
title = "Studying NF-kappa B translocation between nucleus and cytoplasm by electroporative flow cytometry",
abstract = "Transport of protein and RNA cargoes between the nucleus and cytoplasm (nucleocytoplasmic transport) is vital for a variety of cellular functions. In this report, we describe using a novel method, microfluidic electroporative flow cytometry, to study kinetics of nucleocytoplasmic transport of an important transcription factor NF-κB. With data collected from single cells, we quantitatively characterize the population-averaged kinetic parameters such as the rate constants and apparent activation barrier for NF-κB transport. Our data demonstrate that NF-κB nucleocytoplasmic transport fits first-order kinetics very well and is a fairly reversible process governed by equilibrium thermodynamics.",
keywords = "Electroporative flow cytometry, Microfluidic, NF-κB, Nucleocytoplasmic transport, Translocation",
author = "Jun Wang and Bei Fei and Yihong Zhan and Geahlen, \{Robert L.\} and Chang Lu",
year = "2010",
language = "English",
isbn = "9781618390622",
series = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",
pages = "1661--1663",
booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",
note = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 ; Conference date: 03-10-2010 Through 07-10-2010",
}