Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy

C. O.S. Sorzano; A. Jiménez; J. Mota; J. L. Vilas; D. Maluenda; M. Martínez; E. Ramírez-Aportela; T. Majtner; J. Segura; R. Sánchez-García; Y. Rancel; L. Del Caño; P. Conesa; R. Melero; S. Jonic; J. Vargas; F. Cazals; Z. Freyberg; J. Krieger; I. Bahar; R. Marabini; J. M. Carazo

doi:10.1107/S2053230X18015108

Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy

C. O.S. Sorzano
, A. Jiménez
, J. Mota
, J. L. Vilas
, D. Maluenda
, M. Martínez
, E. Ramírez-Aportela
, T. Majtner
, J. Segura
, R. Sánchez-García
, Y. Rancel
, L. Del Caño
, P. Conesa
, R. Melero
, S. Jonic
, J. Vargas
, F. Cazals
, Z. Freyberg
, J. Krieger
, I. Bahar

R. Marabini, J. M. Carazo

Laufer Center for Physical and Quantitative Biology

CSIC - Centro Nacional de Biotecnologia (CNB)
Sorbonne Université
McGill University
INRIA Sophia-Antipolis
University of Pittsburgh
Universidad Autónoma de Madrid

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Single-particle analysis by electron microscopy is a well established technique for analyzing the three-dimensional structures of biological macromolecules. Besides its ability to produce high-resolution structures, it also provides insights into the dynamic behavior of the structures by elucidating their conformational variability. Here, the different image-processing methods currently available to study continuous conformational changes are reviewed.

Original language	English
Pages (from-to)	19-32
Number of pages	14
Journal	Acta Crystallographica Section F: Structural Biology Communications
Volume	75
DOIs	https://doi.org/10.1107/S2053230X18015108
State	Published - 2019

Keywords

continuous heterogeneity
electron microscopy
image processing
normal-mode analysis
single-particle analysis

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10.1107/S2053230X18015108

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Sorzano, C. O. S., Jiménez, A., Mota, J., Vilas, J. L., Maluenda, D., Martínez, M., Ramírez-Aportela, E., Majtner, T., Segura, J., Sánchez-García, R., Rancel, Y., Del Caño, L., Conesa, P., Melero, R., Jonic, S., Vargas, J., Cazals, F., Freyberg, Z., Krieger, J., ... Carazo, J. M. (2019). Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy. Acta Crystallographica Section F: Structural Biology Communications, 75, 19-32. https://doi.org/10.1107/S2053230X18015108

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title = "Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy",

abstract = "Single-particle analysis by electron microscopy is a well established technique for analyzing the three-dimensional structures of biological macromolecules. Besides its ability to produce high-resolution structures, it also provides insights into the dynamic behavior of the structures by elucidating their conformational variability. Here, the different image-processing methods currently available to study continuous conformational changes are reviewed.",

keywords = "continuous heterogeneity, electron microscopy, image processing, normal-mode analysis, single-particle analysis",

author = "Sorzano, \{C. O.S.\} and A. Jim{\'e}nez and J. Mota and Vilas, \{J. L.\} and D. Maluenda and M. Mart{\'i}nez and E. Ram{\'i}rez-Aportela and T. Majtner and J. Segura and R. S{\'a}nchez-Garc{\'i}a and Y. Rancel and \{Del Ca{\~n}o\}, L. and P. Conesa and R. Melero and S. Jonic and J. Vargas and F. Cazals and Z. Freyberg and J. Krieger and I. Bahar and R. Marabini and Carazo, \{J. M.\}",

note = "Publisher Copyright: {\textcopyright} 2019 International Union of Crystallography.",

year = "2019",

doi = "10.1107/S2053230X18015108",

language = "English",

volume = "75",

pages = "19--32",

journal = "Acta Crystallographica Section F: Structural Biology Communications",

issn = "2053-230X",

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Sorzano, COS, Jiménez, A, Mota, J, Vilas, JL, Maluenda, D, Martínez, M, Ramírez-Aportela, E, Majtner, T, Segura, J, Sánchez-García, R, Rancel, Y, Del Caño, L, Conesa, P, Melero, R, Jonic, S, Vargas, J, Cazals, F, Freyberg, Z, Krieger, J, Bahar, I, Marabini, R & Carazo, JM 2019, 'Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy', Acta Crystallographica Section F: Structural Biology Communications, vol. 75, pp. 19-32. https://doi.org/10.1107/S2053230X18015108

TY - JOUR

T1 - Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy

AU - Sorzano, C. O.S.

AU - Jiménez, A.

AU - Mota, J.

AU - Vilas, J. L.

AU - Maluenda, D.

AU - Martínez, M.

AU - Ramírez-Aportela, E.

AU - Majtner, T.

AU - Segura, J.

AU - Sánchez-García, R.

AU - Rancel, Y.

AU - Del Caño, L.

AU - Conesa, P.

AU - Melero, R.

AU - Jonic, S.

AU - Vargas, J.

AU - Cazals, F.

AU - Freyberg, Z.

AU - Krieger, J.

AU - Bahar, I.

AU - Marabini, R.

AU - Carazo, J. M.

PY - 2019

Y1 - 2019

N2 - Single-particle analysis by electron microscopy is a well established technique for analyzing the three-dimensional structures of biological macromolecules. Besides its ability to produce high-resolution structures, it also provides insights into the dynamic behavior of the structures by elucidating their conformational variability. Here, the different image-processing methods currently available to study continuous conformational changes are reviewed.

AB - Single-particle analysis by electron microscopy is a well established technique for analyzing the three-dimensional structures of biological macromolecules. Besides its ability to produce high-resolution structures, it also provides insights into the dynamic behavior of the structures by elucidating their conformational variability. Here, the different image-processing methods currently available to study continuous conformational changes are reviewed.

KW - continuous heterogeneity

KW - electron microscopy

KW - image processing

KW - normal-mode analysis

KW - single-particle analysis

UR - https://www.scopus.com/pages/publications/85059630180

U2 - 10.1107/S2053230X18015108

DO - 10.1107/S2053230X18015108

M3 - Article

C2 - 30605122

AN - SCOPUS:85059630180

SN - 2053-230X

VL - 75

SP - 19

EP - 32

JO - Acta Crystallographica Section F: Structural Biology Communications

JF - Acta Crystallographica Section F: Structural Biology Communications

ER -

Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy

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Keywords

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