Synthesis and characterization of poly(ethylene oxide)/polylactide/polylysine tri-arm star copolymers for gene delivery

Tianyuan Wu; Yu Cai; Xia Zhao; Chai K. Ngai; Benjamin Chu; Benjamin Hsiao; Michael Hadjiargyrou; Robert B. Grubbs

doi:10.1002/pola.28938

Synthesis and characterization of poly(ethylene oxide)/polylactide/polylysine tri-arm star copolymers for gene delivery

Tianyuan Wu
, Yu Cai
, Xia Zhao
, Chai K. Ngai
, Benjamin Chu
, Benjamin Hsiao
, Michael Hadjiargyrou
, Robert B. Grubbs

Chemistry

Stony Brook University
New York Institute of Technology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Amphiphilic cationic poly(ethylene oxide)-S(polylysine)-poly(d,l-lactide) (mPEO-S(CK_n)-PLA) tri-arm star copolymers were synthesized by a combination of ring opening polymerization (ROP) and a thiol–disulfide exchange. The mPEO-S(CK_n)-PLA copolymers were found to be non-cytotoxic and could effectively condense GFP plasmid DNA into nanometer-sized complexes, as characterized by dynamic light scattering (DLS), suitable for endocytotic cellular uptake. In vitro DNA transfection studies showed that the amphiphilic structure is capable of DNA transfection and GFP expression. Addition of chloroquine into the medium further enhanced the DNA transfection efficiency.

Original language	English
Pages (from-to)	635-644
Number of pages	10
Journal	Journal of Polymer Science, Part A: Polymer Chemistry
Volume	56
Issue number	6
DOIs	https://doi.org/10.1002/pola.28938
State	Published - Mar 15 2018

Keywords

gene transfection
ring-opening polymerization
star copolymers

Access to Document

10.1002/pola.28938

Cite this

@article{b7a00209714f4693aac3459c88e493cb,

title = "Synthesis and characterization of poly(ethylene oxide)/polylactide/polylysine tri-arm star copolymers for gene delivery",

abstract = "Amphiphilic cationic poly(ethylene oxide)-S(polylysine)-poly(d,l-lactide) (mPEO-S(CKn)-PLA) tri-arm star copolymers were synthesized by a combination of ring opening polymerization (ROP) and a thiol–disulfide exchange. The mPEO-S(CKn)-PLA copolymers were found to be non-cytotoxic and could effectively condense GFP plasmid DNA into nanometer-sized complexes, as characterized by dynamic light scattering (DLS), suitable for endocytotic cellular uptake. In vitro DNA transfection studies showed that the amphiphilic structure is capable of DNA transfection and GFP expression. Addition of chloroquine into the medium further enhanced the DNA transfection efficiency.",

keywords = "gene transfection, ring-opening polymerization, star copolymers",

author = "Tianyuan Wu and Yu Cai and Xia Zhao and Ngai, \{Chai K.\} and Benjamin Chu and Benjamin Hsiao and Michael Hadjiargyrou and Grubbs, \{Robert B.\}",

note = "Publisher Copyright: {\textcopyright} 2017 Wiley Periodicals, Inc.",

year = "2018",

month = mar,

day = "15",

doi = "10.1002/pola.28938",

language = "English",

volume = "56",

pages = "635--644",

journal = "Journal of Polymer Science, Part A: Polymer Chemistry",

issn = "0887-624X",

number = "6",

}

TY - JOUR

T1 - Synthesis and characterization of poly(ethylene oxide)/polylactide/polylysine tri-arm star copolymers for gene delivery

AU - Wu, Tianyuan

AU - Cai, Yu

AU - Zhao, Xia

AU - Ngai, Chai K.

AU - Chu, Benjamin

AU - Hsiao, Benjamin

AU - Hadjiargyrou, Michael

AU - Grubbs, Robert B.

PY - 2018/3/15

Y1 - 2018/3/15

N2 - Amphiphilic cationic poly(ethylene oxide)-S(polylysine)-poly(d,l-lactide) (mPEO-S(CKn)-PLA) tri-arm star copolymers were synthesized by a combination of ring opening polymerization (ROP) and a thiol–disulfide exchange. The mPEO-S(CKn)-PLA copolymers were found to be non-cytotoxic and could effectively condense GFP plasmid DNA into nanometer-sized complexes, as characterized by dynamic light scattering (DLS), suitable for endocytotic cellular uptake. In vitro DNA transfection studies showed that the amphiphilic structure is capable of DNA transfection and GFP expression. Addition of chloroquine into the medium further enhanced the DNA transfection efficiency.

AB - Amphiphilic cationic poly(ethylene oxide)-S(polylysine)-poly(d,l-lactide) (mPEO-S(CKn)-PLA) tri-arm star copolymers were synthesized by a combination of ring opening polymerization (ROP) and a thiol–disulfide exchange. The mPEO-S(CKn)-PLA copolymers were found to be non-cytotoxic and could effectively condense GFP plasmid DNA into nanometer-sized complexes, as characterized by dynamic light scattering (DLS), suitable for endocytotic cellular uptake. In vitro DNA transfection studies showed that the amphiphilic structure is capable of DNA transfection and GFP expression. Addition of chloroquine into the medium further enhanced the DNA transfection efficiency.

KW - gene transfection

KW - ring-opening polymerization

KW - star copolymers

UR - https://www.scopus.com/pages/publications/85039154303

U2 - 10.1002/pola.28938

DO - 10.1002/pola.28938

M3 - Article

AN - SCOPUS:85039154303

SN - 0887-624X

VL - 56

SP - 635

EP - 644

JO - Journal of Polymer Science, Part A: Polymer Chemistry

JF - Journal of Polymer Science, Part A: Polymer Chemistry

IS - 6

ER -

Synthesis and characterization of poly(ethylene oxide)/polylactide/polylysine tri-arm star copolymers for gene delivery

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this