TY - GEN
T1 - Tuning field non-uniformity across microchannels for flow-through dielectrophoretic separations
AU - Huang, Xu Hai
AU - Torres-Castro, Karina
AU - Varhue, Walter
AU - Swami, Nathan S.
N1 - Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - Microfluidic separation and biophysical characterization of cells based on their distinct electrophysiology is widely carried out based on dielectrophoretic translation of cells. However, throughputs have been low due to the limited field extent and field non-uniformity obtained from 2D electrodes in microchannels. We present a strategy for co-fabrication of a set of electrode and sample channels that can be filled sequentially to create recessed 3D metal edges, with an ability to modulate the field non-uniformity across the sample microchannel, so that enabling positive and negative dielectrophoretic separations to be carried out at a high cell throughput of 2.2 × 104 cells/min.
AB - Microfluidic separation and biophysical characterization of cells based on their distinct electrophysiology is widely carried out based on dielectrophoretic translation of cells. However, throughputs have been low due to the limited field extent and field non-uniformity obtained from 2D electrodes in microchannels. We present a strategy for co-fabrication of a set of electrode and sample channels that can be filled sequentially to create recessed 3D metal edges, with an ability to modulate the field non-uniformity across the sample microchannel, so that enabling positive and negative dielectrophoretic separations to be carried out at a high cell throughput of 2.2 × 104 cells/min.
KW - 3D electrode in microchannel
KW - Co-fabrication
KW - Dielectrophoresis
UR - https://www.scopus.com/pages/publications/85098243641
M3 - Conference contribution
AN - SCOPUS:85098243641
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 340
EP - 341
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -