Two-photon fiber STED microscope demonstrated at depth in tissue phantom

Catherine A. Saladrigas; Raymond Anchordoquy; Matthew Heyrich; Diego Restrepo; Mark E. Siemens; Emily A. Gibson; Juliet T. Gopinath

doi:10.1364/CLEO_SI.2025.SS144_2

Two-photon fiber STED microscope demonstrated at depth in tissue phantom

Catherine A. Saladrigas
, Raymond Anchordoquy
, Matthew Heyrich
, Diego Restrepo
, Mark E. Siemens
, Emily A. Gibson
, Juliet T. Gopinath

Physiology and Biophysics

University of Colorado Boulder
University of Denver
University of Colorado Denver

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We present the first demonstration of resolution enhancement at depth in a tissue phantom using a fiber-based depletion beam with two-photon excitation.

Original language	English
Title of host publication	CLEO
Subtitle of host publication	Science and Innovations - Proceedings CLEO 2025
Publisher	Optical Society of America
ISBN (Electronic)	9781957171500
DOIs	https://doi.org/10.1364/CLEO_SI.2025.SS144_2
State	Published - 2025
Event	2025 CLEO: Science and Innovations, CLEO-SI 2025 - Long Beach, United States Duration: May 4 2025 → May 9 2025

Publication series

Name	CLEO: Science and Innovations - Proceedings CLEO 2025

Conference

Conference	2025 CLEO: Science and Innovations, CLEO-SI 2025
Country/Territory	United States
City	Long Beach
Period	05/4/25 → 05/9/25

Access to Document

10.1364/CLEO_SI.2025.SS144_2

Cite this

Saladrigas, C. A., Anchordoquy, R., Heyrich, M., Restrepo, D., Siemens, M. E., Gibson, E. A., & Gopinath, J. T. (2025). Two-photon fiber STED microscope demonstrated at depth in tissue phantom. In CLEO: Science and Innovations - Proceedings CLEO 2025 (CLEO: Science and Innovations - Proceedings CLEO 2025). Optical Society of America. https://doi.org/10.1364/CLEO_SI.2025.SS144_2

@inproceedings{b73e5b2712c542e3bae258b8523bc59b,

title = "Two-photon fiber STED microscope demonstrated at depth in tissue phantom",

abstract = "We present the first demonstration of resolution enhancement at depth in a tissue phantom using a fiber-based depletion beam with two-photon excitation.",

author = "Saladrigas, \{Catherine A.\} and Raymond Anchordoquy and Matthew Heyrich and Diego Restrepo and Siemens, \{Mark E.\} and Gibson, \{Emily A.\} and Gopinath, \{Juliet T.\}",

note = "Publisher Copyright: {\textcopyright} Optica Publishing Group 2025 {\textcopyright} 2024 The Author(s); 2025 CLEO: Science and Innovations, CLEO-SI 2025 ; Conference date: 04-05-2025 Through 09-05-2025",

year = "2025",

doi = "10.1364/CLEO\_SI.2025.SS144\_2",

language = "English",

series = "CLEO: Science and Innovations - Proceedings CLEO 2025",

publisher = "Optical Society of America",

booktitle = "CLEO",

}

Saladrigas, CA, Anchordoquy, R, Heyrich, M, Restrepo, D, Siemens, ME, Gibson, EA & Gopinath, JT 2025, Two-photon fiber STED microscope demonstrated at depth in tissue phantom. in CLEO: Science and Innovations - Proceedings CLEO 2025. CLEO: Science and Innovations - Proceedings CLEO 2025, Optical Society of America, 2025 CLEO: Science and Innovations, CLEO-SI 2025, Long Beach, United States, 05/4/25. https://doi.org/10.1364/CLEO_SI.2025.SS144_2

Two-photon fiber STED microscope demonstrated at depth in tissue phantom. / Saladrigas, Catherine A.; Anchordoquy, Raymond; Heyrich, Matthew et al.
CLEO: Science and Innovations - Proceedings CLEO 2025. Optical Society of America, 2025. (CLEO: Science and Innovations - Proceedings CLEO 2025).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Two-photon fiber STED microscope demonstrated at depth in tissue phantom

AU - Saladrigas, Catherine A.

AU - Anchordoquy, Raymond

AU - Heyrich, Matthew

AU - Restrepo, Diego

AU - Siemens, Mark E.

AU - Gibson, Emily A.

AU - Gopinath, Juliet T.

PY - 2025

Y1 - 2025

N2 - We present the first demonstration of resolution enhancement at depth in a tissue phantom using a fiber-based depletion beam with two-photon excitation.

AB - We present the first demonstration of resolution enhancement at depth in a tissue phantom using a fiber-based depletion beam with two-photon excitation.

UR - https://www.scopus.com/pages/publications/105033856072

U2 - 10.1364/CLEO_SI.2025.SS144_2

DO - 10.1364/CLEO_SI.2025.SS144_2

M3 - Conference contribution

AN - SCOPUS:105033856072

T3 - CLEO: Science and Innovations - Proceedings CLEO 2025

BT - CLEO

PB - Optical Society of America

T2 - 2025 CLEO: Science and Innovations, CLEO-SI 2025

Y2 - 4 May 2025 through 9 May 2025

ER -

Two-photon fiber STED microscope demonstrated at depth in tissue phantom

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