Two-photon microendoscope for label-free imaging in stereotactic neurosurgery

Tarah A. Welton; Nicholas M. George; Baris N. Ozbay; Arianna Gentile Polese; Gregory Osborne; Gregory L. Futia; J. Keenan Kushner; Bette Kleinschmidt-Demasters; Allyson L. Alexander; Aviva Abosch; Steven Ojemann; Diego Restrepo; Emily A. Gibson

doi:10.1364/BOE.492552

Two-photon microendoscope for label-free imaging in stereotactic neurosurgery

Tarah A. Welton
, Nicholas M. George
, Baris N. Ozbay
, Arianna Gentile Polese
, Gregory Osborne
, Gregory L. Futia
, J. Keenan Kushner
, Bette Kleinschmidt-Demasters
, Allyson L. Alexander
, Aviva Abosch
, Steven Ojemann
, Diego Restrepo
, Emily A. Gibson

Physiology and Biophysics

University of Colorado Denver
University of Colorado Anschutz Medical Campus
Intelligent Imaging Innovations, Inc.
University of Nebraska Medical Center

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We demonstrate a gradient refractive index (GRIN) microendoscope with an outer diameter of ∼1.2 mm and a length of ∼186 mm that can fit into a stereotactic surgical cannula. Two photon imaging at an excitation wavelength of 900 nm showed a field of view of ∼180 microns and a lateral and axial resolution of 0.86 microns and 9.6 microns respectively. The microendoscope was tested by imaging autofluorescence and second harmonic generation (SHG) in label-free human brain tissue. Furthermore, preliminary image analysis indicates that image classification models can predict if an image is from the subthalamic nucleus or the surrounding tissue using conventional, bench-top two-photon autofluorescence.

Original language	English
Pages (from-to)	3705-3725
Number of pages	21
Journal	Biomedical Optics Express
Volume	14
Issue number	7
DOIs	https://doi.org/10.1364/BOE.492552
State	Published - 2023

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title = "Two-photon microendoscope for label-free imaging in stereotactic neurosurgery",

abstract = "We demonstrate a gradient refractive index (GRIN) microendoscope with an outer diameter of ∼1.2 mm and a length of ∼186 mm that can fit into a stereotactic surgical cannula. Two photon imaging at an excitation wavelength of 900 nm showed a field of view of ∼180 microns and a lateral and axial resolution of 0.86 microns and 9.6 microns respectively. The microendoscope was tested by imaging autofluorescence and second harmonic generation (SHG) in label-free human brain tissue. Furthermore, preliminary image analysis indicates that image classification models can predict if an image is from the subthalamic nucleus or the surrounding tissue using conventional, bench-top two-photon autofluorescence.",

author = "Welton, \{Tarah A.\} and George, \{Nicholas M.\} and Ozbay, \{Baris N.\} and Polese, \{Arianna Gentile\} and Gregory Osborne and Futia, \{Gregory L.\} and Kushner, \{J. Keenan\} and Bette Kleinschmidt-Demasters and Alexander, \{Allyson L.\} and Aviva Abosch and Steven Ojemann and Diego Restrepo and Gibson, \{Emily A.\}",

note = "Publisher Copyright: {\textcopyright} 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.",

year = "2023",

doi = "10.1364/BOE.492552",

language = "English",

volume = "14",

pages = "3705--3725",

journal = "Biomedical Optics Express",

issn = "2156-7085",

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TY - JOUR

T1 - Two-photon microendoscope for label-free imaging in stereotactic neurosurgery

AU - Welton, Tarah A.

AU - George, Nicholas M.

AU - Ozbay, Baris N.

AU - Polese, Arianna Gentile

AU - Osborne, Gregory

AU - Futia, Gregory L.

AU - Kushner, J. Keenan

AU - Kleinschmidt-Demasters, Bette

AU - Alexander, Allyson L.

AU - Abosch, Aviva

AU - Ojemann, Steven

AU - Restrepo, Diego

AU - Gibson, Emily A.

PY - 2023

Y1 - 2023

N2 - We demonstrate a gradient refractive index (GRIN) microendoscope with an outer diameter of ∼1.2 mm and a length of ∼186 mm that can fit into a stereotactic surgical cannula. Two photon imaging at an excitation wavelength of 900 nm showed a field of view of ∼180 microns and a lateral and axial resolution of 0.86 microns and 9.6 microns respectively. The microendoscope was tested by imaging autofluorescence and second harmonic generation (SHG) in label-free human brain tissue. Furthermore, preliminary image analysis indicates that image classification models can predict if an image is from the subthalamic nucleus or the surrounding tissue using conventional, bench-top two-photon autofluorescence.

AB - We demonstrate a gradient refractive index (GRIN) microendoscope with an outer diameter of ∼1.2 mm and a length of ∼186 mm that can fit into a stereotactic surgical cannula. Two photon imaging at an excitation wavelength of 900 nm showed a field of view of ∼180 microns and a lateral and axial resolution of 0.86 microns and 9.6 microns respectively. The microendoscope was tested by imaging autofluorescence and second harmonic generation (SHG) in label-free human brain tissue. Furthermore, preliminary image analysis indicates that image classification models can predict if an image is from the subthalamic nucleus or the surrounding tissue using conventional, bench-top two-photon autofluorescence.

UR - https://www.scopus.com/pages/publications/85165447377

U2 - 10.1364/BOE.492552

DO - 10.1364/BOE.492552

M3 - Article

AN - SCOPUS:85165447377

SN - 2156-7085

VL - 14

SP - 3705

EP - 3725

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 7

ER -

Two-photon microendoscope for label-free imaging in stereotactic neurosurgery

Abstract

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